Restriction of rapid eye movement sleep during adolescence increases energy gain and metabolic efficiency in young adult rats

Author(s)

Ribeiro-Silva, Neila ^[1] ; Nejm, Mariana Bocca ^[1] ; Affonso da Silva, Sylvia Maria ^[1] ; Suchecki, Deborah ^[2] ; Luz, Jacqueline ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Physiol, Rua Napoleao De Barros 925, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Psychobiol, Rua Napoleao De Barros 925, BR-04023062 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Experimental Physiology; v. 101, n. 2, p. 308-318, FEB 1 2016.
• Web of Science Citations: 2

Abstract

New Findings What is the central question of this study? Sleep curtailment in infancy and adolescence may lead to long-term risk for obesity, but the mechanisms involved have not yet been determined. This study examined the immediate and long-term metabolic effects produced by sleep restriction in young rats. What is the main finding and its importance? Prolonged sleep restriction reduced weight gain (body fat stores) in young animals. After prolonged recovery, sleep-restricted rats tended to save more energy and to store more fat, possibly owing to increased gross food efficiency. This could be the first step to understand this association. Sleep curtailment is associated with obesity and metabolic changes in adults and children. The aim of the present study was to evaluate the immediate and long-term metabolic alterations produced by sleep restriction in pubertal male rats. Male Wistar rats (28days old) were allocated to a control (CTL) group or a sleep-restricted (SR) group. This was accomplished by the single platform technique for 18h per day for 21 days. These groups were subdivided into the following four time points for assessment: sleep restriction and 1, 2 and 4 months of recovery. Body weight and food intake were monitored throughout the experiment. At the end of each time period, blood was collected for metabolic profiling, and the carcasses were processed for measurement of body composition and energy balance. During the period of sleep restriction, SR animals consumed less food in the home cages. This group also displayed lower body weight, body fat, triglycerides and glucose levels than CTL rats. At the end of the first month of recovery, despite eating as much as CTL rats, SR animals showed greater energy and body weight gain, increased gross food efficiency and decreased energy expenditure. At the end of the second and fourth months of recovery, the groups were no longer different, except for energy gain and gross food efficiency, which remained higher in SR animals. In conclusion, sleep restriction affected weight gain of young animals, owing to reduction of fat stores. Two months were sufficient to recover this deficit and to reveal that SR rats tended to save more energy and to store more fat. (AU)

• FAPESP's process: 10/51192-9 - Chronic sleep restriction in the post-weaning: later effects on metabolism
• Grantee: Jacqueline Luz
• Support Opportunities: Regular Research Grants