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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cyanobacteria from Terrestrial and Marine Sources Contain Apoptogens Able to Overcome Chemoresistance in Acute Myeloid Leukemia Cells

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Author(s):
Liu, Liwei [1] ; Herfindal, Lars [2, 3] ; Jokela, Jouni [1] ; Shishido, Tania Keiko [1] ; Wahlsten, Matti [1] ; Doskeland, Stein Ove [2] ; Sivonen, Kaarina [1]
Total Authors: 7
Affiliation:
[1] Univ Helsinki, Div Microbiol & Biotechnol, Dept Food & Environm Sci, Helsinki 00014 - Finland
[2] Univ Bergen, Dept Biomed, N-5009 Bergen - Norway
[3] Haukeland Hosp, Translat Signaling Grp, N-5009 Bergen - Norway
Total Affiliations: 3
Document type: Journal article
Source: MARINE DRUGS; v. 12, n. 4, p. 2036-2053, APR 2014.
Web of Science Citations: 4
Abstract

In this study, we investigated forty cyanobacterial isolates from biofilms, gastropods, brackish water and symbiotic lichen habitats. Their aqueous and organic extracts were used to screen for apoptosis-inducing activity against acute myeloid leukemia cells. A total of 28 extracts showed cytotoxicity against rat acute myeloid leukemia (IPC-81) cells. The design of the screen made it possible to eliminate known toxins, such as microcystins and nodularin, or known metabolites with anti-leukemic activity, such as adenosine and its analogs. A cytotoxicity test on human embryonic kidney (HEK293T) fibroblasts indicated that 21 of the 28 extracts containing anti-acute myeloid leukemia (AML) activity showed selectivity in favor of leukemia cells. Extracts L26-O and L30-O were able to partly overcome the chemotherapy resistance induced by the oncogenic protein Bcl-2, whereas extract L1-O overcame protection from the deletion of the tumor suppressor protein p53. In conclusion, cyanobacteria are a prolific resource for anti-leukemia compounds that have potential for pharmaceutical applications. Based on the variety of cellular responses, we also conclude that the different anti-leukemic compounds in the cyanobacterial extracts target different elements of the death machinery of mammalian cells. (AU)

FAPESP's process: 09/13455-0 - Cyanobacteria from the caatinga biome: isolation, morphology, phylogeny and bioprospection
Grantee:Tânia Keiko Shishido
Support Opportunities: Scholarships in Brazil - Doctorate