Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Triiodothyronine Rapidly Alters the TSH Content and the Secretory Granules Distribution in Male Rat Thyrotrophs by a Cytoskeleton Rearrangement-Independent Mechanism

Full text
Author(s):
Bargi-Souza, Paula [1] ; Romano, Renata Marino [1] ; Salgado, Renato de Mayrinck [2] ; Goulart-Silva, Francemilson [1] ; Brunetto, Erika Lia [1] ; Tenorio Zorn, Telma Maria [2] ; Nunes, Maria Tereza [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Endocrinology; v. 154, n. 12, p. 4908-4918, DEC 2013.
Web of Science Citations: 14
Abstract

Rapid actions of T-3 on TSH synthesis in posttranscriptional steps, such as polyadenylation and translation rate, have already been described. The focus of this paper was to characterize rapid actions of T-3 on TSH secretion and the involvement of actin and microtubule cytoskeleton in this process. For that, sham-operated (SO) and thyroidectomized (Tx) rats were subjected to acute or chronic treatment with T-3. We observed a disarrangement in microtubule and actin cytoskeletons and an increase in Tshb mRNA levels in Tx rats, whereas the total TSH protein content was reduced in the pituitary gland as a whole, but increased in the secretory granules close to the plasma membrane of thyrotrophs, as well as in the extracellular space. The acute T-3 dose promoted a rapid increase and redistribution of TSH secretory granules throughout the cytoplasm, as well as a rearrangement in actin and microtubule cytoskeletons. The T-3 chronic treatment outcome reinforces the acute effects observed and, additionally, evinces an increase in the alpha-tubulin content and a rearrangement in microtubule cytoskeleton. Thus, T-3 is able to rapidly suppress TSH secretion and, in parallel, to promote a rearrangement in actin and microtubules assembly throughout the pituitary gland, effects that seem to be independent from each other. (AU)