Determination of Amphetamine, Amfepramone and Fenproporex in Urine Samples by HPLC-DAD: Application to a Population of Brazilian Truck Drivers

Author(s)

Takitane, Juliana ^[1] ; Almeida, Rafael M. ^[2] ; Oliveira, Tiago F. ^[2] ; Prado, Natanael V. ^[3] ; Munoz, DanielR. ^[1] ; Leyton, Vilma ^[1] ; Yonamine, Mauricio ^[2]

Affiliation

^[1] Univ Sao Paulo, Fac Med, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo, SP - Brazil

^[3] Minist Justica, Dept Policia Rodoviaria Fed, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 27, n. 3, p. 624-630, MAR 2016.
• Web of Science Citations: 1

Abstract

Commercially available immunoassay tests are designed to detect the presence of amphetamine/methamphetamine or methylenodioxyamphetamines. However, it is known that Brazilian truck drivers also report the use of other illicit amphetamines, such as amfepramone and fenproporex. Thus, a method was developed and validated in order to quantify amphetamine-type stimulants (amphetamine, fenproporex and amfepramone) in urine by high performance liquid chromatography with diode array detection (HPLC-DAD). Prior to this, a liquid-liquid extraction (LLE) with diethyl ether was performed in order to extract the analytes. The limit of detection was 150 ng mL-1. The method showed to be precise (relative standard deviation, RSD < 15%) and the recovery values for the three analytes were greater than 50%. The linearity ranged from 150 to 1000 ng mL-1(r2 > 0.99). Urine samples randomly collected from 385 truck drivers in Brazilian roads were submitted to the developed method. Nine samples were tested positive for amphetamine and one was tested positive for fenproporex and amphetamine. (AU)

• FAPESP's process: 11/02848-1 - Assessment of amphetamine use by professional drivers through urine toxicological analysis
• Grantee: Juliana Takitane
• Support Opportunities: Scholarships in Brazil - Master