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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Apoptosis in Early Salivary Gland Duct Morphogenesis and Lumen Formation

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Author(s):
Teshima, T. H. N. [1] ; Wells, K. L. [2] ; Lourenco, S. V. [1] ; Tucker, A. S. [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil
[2] Kings Coll London, Dept Craniofacial Dev & Stem Cell Biol, London SE1 9RT - England
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF DENTAL RESEARCH; v. 95, n. 3, p. 277-283, MAR 2016.
Web of Science Citations: 11
Abstract

Salivary glands are essential for the maintenance of oral health by providing lubrication and antimicrobial protection to the mucosal and tooth surfaces. Saliva is modified and delivered to the oral cavity by a complex multifunctional ductal system. During development, these ducts form as solid tubes, which undergo cavitation to create lumens. Apoptosis has been suggested to play a role in this cavitation process along with changes in cell polarity. Here, we show that apoptosis occurs from the very earliest stages of mouse salivary gland development, much earlier than previously reported. Apoptotic cells were observed in the center of the first epithelial stalk at earlystage embryonic day 12.5 (E12.5) according to both TUNEL staining and cleaved caspase 3 immunofluorescence. The presumptive lumen space was highlighted by the colocalization of a predictive lumen marker, cytokeratin 7. At E14.5, as lumens start to form throughout the glands, apoptotic expression decreased while cytokeratin 7 remained positive. In vitro inhibition of all caspases in E12.5 and E13.5 salivary glands resulted in wider ducts, as compared with the controls, and a defect in lumen formation. In contrast, no such defect in lumen formation was observed at E14.5. Our data indicate that apoptosis is involved during early stages of gland formation (E12.5 onward) and appears important for shaping the forming ducts. (AU)

FAPESP's process: 11/18865-2 - Investigation of apoptotic activity in the lumen formation of salivary gland ducts: comparative analysis between animal and human based models
Grantee:Tathyane Harumi Nakajima Teshima
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)