| Full text | |
| Author(s): |
Calil-Silveira, Jamile
[1]
;
Serrano-Nascimento, Caroline
[1]
;
Kopp, Peter Andreas
[2]
;
Nunes, Maria Tereza
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo, SP - Brazil
[2] Northeastern Univ, Feinberg Sch Med, Div Endocrinol Metab & Mol Med, Chicago, IL - USA
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY; v. 310, n. 7, p. C576-C582, APR 1 2016. |
| Web of Science Citations: | 5 |
| Abstract | |
Adequate iodide supply and metabolism are essential for thyroid hormones synthesis. In thyrocytes, iodide uptake is mediated by the sodium-iodide symporter, but several proteins appear to be involved in iodide efflux. Previous studies demonstrated that pendrin is able to mediate apical efflux of iodide in thyrocytes. Acute iodide excess transiently impairs thyroid hormone synthesis, a phenomenon known as the Wolff-Chaikoff effect. Although the escape from this inhibitory effect is not completely understood, it has been related to the inhibition of sodium-iodide symporter-mediated iodide uptake. However, the effects of iodide excess on iodide efflux have not been characterized. Herein, we investigated the consequences of iodide excess on pendrin abundance, subcellular localization, and iodide efflux in rat thyroid PCC13 cells. Our results indicate that iodide excess increases pendrin abundance and plasma membrane insertion after 24 h of treatment. Moreover, iodide excess increases pendrin half-life. Finally, iodide exposure also increases iodide efflux from PCC13 cells. In conclusion, these data suggest that pendrin may have an important role in mediating iodide efflux in thyrocytes, especially under conditions of iodide excess. (AU) | |
| FAPESP's process: | 12/24391-6 - Impact of iodine excess exposure during pregnancy and/or lactation on the hypothalamus-pituitary-thyroid axis activity in rat dams and their offspring |
| Grantee: | Caroline Serrano Do Nascimento |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/05503-2 - EFFECT OF ACUTE ADMINISTRATION OF IODINE IN THE PENDRIN GENE EXPRESSION AND PROMOTER ACTIVITY: STUDY IN VIVO AND IN VITRO |
| Grantee: | Jamile Calil Silveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 09/17834-6 - Study of the molecular basis of the short-term regulation of the Na+/I- symporter (NIS) and pendrin gene expression in rats thyroid and PCCl3 cells: possible contribution to the comprehension of the Wolff-Chaikoff effect and the escape phenomenon |
| Grantee: | Maria Tereza Nunes |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/13011-8 - Does iodide regulate the expression and activity of its apical transporter, pendrin? Study in PCCl3 and TSA-201 cells |
| Grantee: | Jamile Calil Silveira |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |