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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bone repair of critical size defects treated with autogenic, allogenic, or xenogenic bone grafts alone or in combination with rhBMP-2

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Author(s):
Issa, Joao Paulo [1] ; Gonzaga, Miliane [2] ; Kotake, Bruna Gabriela [2] ; de Lucia, Conrado [1] ; Ervolino, Edilson [3] ; Iyomasa, Mamie [4]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Morphol Physiol & Basic Pathol, Av Cafe S-N Ribeirao Preto, Sao Paulo - Brazil
[2] Univ Sao Paulo, Locomotor Sch Med Ribeirao Preto, Dept Biomech Med & Rehabil, Sao Paulo - Brazil
[3] Sao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Dent, Dept Morphol Physiol & Basic Pathol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Clinical Oral Implants Research; v. 27, n. 5, p. 558-566, MAY 2016.
Web of Science Citations: 13
Abstract

ObjectiveTo evaluate the bone repair of critical size defects treated with autogenic, allogenic, or xenogenic bone grafts alone or in combination with rhBMP-2. Material and methodsIn 112 rats, a critical bone defect of 5mm bilaterally in the calvaria was made and filled with different bone grafts alone or combined with rhBMP-2: group autograft (AuG); group allograft (AlG); group xenograft (XeG); group AuG/BMP-2 (autograft and 5g rhBMP-2); group AlG/BMP-2 (allograft and 5g rhBMP-2); group XeG/BMP-2 (xenograft and 5g rhBMP-2); group BMP-2 (5g rhBMP-2); and control group, filled only with blood coagulum. After a period of 4 or 6weeks, the animals were euthanized. Histological and histometric analyses were performed for new bone formation (NB), as well as the immunohistochemical detection of tartrate-resistant acid phosphatase (TRAP), and zymographic analysis (MMP) of type 2 and 9. ResultsHistological analysis showed the bone healing process was faster and favorable in AuG and AuG/BMP-2. In both periods, the grafted groups (AuG, XeG and AlG) had a greater volume of NB than the control group, which was even greater when combined with rhBMP-2. The XeG group showed a higher number of TRAP-positive multinucleated osteoclasts, and enzymatic activity revealed different levels of proMMP-2, MMP-2, and MMP-9 (6weeks). ConclusionThe different types of grafts increased bone formation, mainly associated with rhBMP-2, enhancing and accelerating the repair process. These groups had higher enzymatic indices than the control group especially with XeG, which also showed higher TRAP-positive multinucleated cells similar to osteoclasts, suggesting a remodeling process. (AU)

FAPESP's process: 11/01512-0 - Evaluation on the interaction between two osteogenic proteins and different bone grafts in the bone healing process of calvarial tissue in Wistar rats
Grantee:João Paulo Mardegan Issa
Support Opportunities: Regular Research Grants