| Full text | |
| Author(s): |
Milazzotto, Marcella Pecora
[1]
;
Goissis, Marcelo Demarchi
[2]
;
Chitwood, James Lee
[3]
;
Annes, Kelly
[1]
;
Soares, Carlos Alexandre
[1]
;
Ispada, Jessica
[1]
;
Ortiz Avila Assumpcao, Mayra Elena
[2]
;
Ross, Pablo Juan
[3]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed ABC, Ctr Nat & Human Sci, Rua Santa Adelia, 166 T3, BlA, S642, BR-09210190 Santo Andre, SP - Brazil
[2] Univ Sao Paulo, Dept Anim Reprod, Sao Paulo - Brazil
[3] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Molecular Reproduction and Development; v. 83, n. 4, p. 324-336, APR 2016. |
| Web of Science Citations: | 11 |
| Abstract | |
Embryo morphokinetics suggests that the timing of the first embryonic cell divisions may predict the developmental potential of an embryo; however, correlations between embryonic morphokinetics and physiology are not clear. Here, we used RNA sequencing to determine the gene expression profile of in vitro-produced early- and late-dividing bovine embryos and their respective blastocysts, and compared these profiles to in vivo-produced blastocysts to identify differentially expressed genes (DEGs). Principal component analysis revealed that fast- and slow-dividing embryos possess similar transcript abundance over the first cleavages. By the blastocyst stage, however, more DEGs were observed between the fast- and slow-dividing embryo groups, whereas blastocysts from the slow-dividing group were more similar to in vivo-produced blastocysts. Gene ontology enrichment analysis showed that the slow-dividing and in vivo-produced blastocysts shared biological processes related to groups of up- or down-regulated genes when compared to the fast-dividing blastocysts. Based on these DEG results, we characterized the relationship between developmental kinetics and energy metabolism of in vitro-produced bovine embryos. Embryos from fast- and slow-dividing groups exhibited different pyruvate and lactate metabolism at 22hr post-in vitro culture (hpc), glucose consumption at 96hpc, and glutamate metabolism at 168hpc. Glycogen storage was similar between cleavage-stage and morulae groups, but was higher in the blastocysts of the slow-dividing group. On the other hand, blastocysts of the fast-dividing group had a higher concentration of lipids. Taken together, these data identify transcriptomic and metabolic differences between embryos with different morphokinetics, suggesting that sorting embryos based on cleavage speed may select for different metabolic patterns. Mol. Reprod. Dev. 83: 324-336, 2016. (c) 2016 Wiley Periodicals, Inc. (AU) | |
| FAPESP's process: | 13/13199-0 - LIPID METABOLISM DURING THE INITIAL DEVELOPMENT OF IN VITRO PRODUCED BOVINE EMBRYOS |
| Grantee: | Kelly Annes |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/20535-3 - Energy metabolism of in vitro produced bovine embryos and its relationship to embryo quality. |
| Grantee: | Carlos Alexandre Soares |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/10351-2 - Metabolomics for non-invasive evaluation of in vitro produced bovine embryos |
| Grantee: | Marcella Pecora Milazzotto |
| Support Opportunities: | Regular Research Grants |