XPC (A2920C), XPF (T30028C), TP53 (Arg72Pro), and GSTP1 (Ile105Val) polymorphisms in prognosis of cutaneous melanoma

Author(s)

Boas Gomez, Gabriela Vilas ^[1] ; de Oliveira, Cristiane ^[1] ; Rinck-Junior, Jose Augusto ^[1] ; de Moraes, Aparecida Machado ^[1] ; Lourenco, Gustavo Jacob ^[1] ; Passos Lima, Carmen Silvia ^[1]

Affiliation

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, Rua Alexander Fleming, 181 Barao Geraldo, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: TUMOR BIOLOGY; v. 37, n. 3, p. 3163-3171, MAR 2016.
• Web of Science Citations: 2

Abstract

This study aimed to evaluate whether XPC A2920C, XPF T30028C, TP53 Arg72Pro, and GSTP1 Ile105Val polymorphisms alter outcomes of cutaneous melanoma (CM) patients. DNA from 237 CM patients seen at the University of Campinas Teaching Hospital from April 2000 to February 2014 was analyzed by polymerase chain reaction and restriction fragment length polymorphism assays. The prognostic impact of genotypes of polymorphisms on progression-free survival (PFS) and overall survival (OS) of CM patients were examined using the Kaplan-Meier probability estimates and univariate and multivariate Cox regression analyses. At 60 months of follow-up, shorter PFS and OS were seen in patients with XPF CC genotype (48.9 vs. 66.7 %, P = 0.002; 77.9 vs. 83.5 %, P = 0.006, respectively) and XPF CC + TP53 ArgArg (43.6 vs. 65.9 %, P = 0.007; 71.6 vs. 84.8 %, P = 0.006, respectively) compared with those with remaining genotypes (Kaplan-Meier estimates). Patients with XPF CC (hazard ratio (HR) 2.45, P = 0.002; HR 3.77, P = 0.005) and XPF CC + TP53 ArgArg (HR 2.67, P = 0.009; HR 4.04, P = 0.03) genotypes had more chance to present tumor progression in univariate and multivariate analyses, whereas patients with XPF CC (HR 2.78, P = 0.009) and XPF CC + TP53 ArgArg (HR 3.84, P = 0.01) genotypes were under greater risk of progressing to death in univariate analysis, compared with those with the remaining genotypes. The data suggest, for the first time, that inherited abnormalities in DNA repair pathway related to XPF 30028C and TP53 Arg72Pro polymorphisms act as prognostic factors for PFS and OS of CM patients. (AU)

• FAPESP's process: 10/18904-5 - The influence of the p53 arg72pro, Mdm2 t309g, Bcl2 c938a and Bax g248a, genetic polymorphisms, involved in induction of apoptosis, in the malignant melanoma risk
• Grantee: Carmen Silvia Passos Lima
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/10042-5 - Influence of the PDCD1 polymorphisms, related to activity of T lymphocytes, in gene expression and susceptibility to cutaneous melanoma
• Grantee: Gabriela Vilas Bôas Gomez
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/12602-0 - Influence of the polymorphisms TP53 arg72pro, MDM2 t309g, Bcl2 c938a e Bax g248a, related with celular apoptosis, in the susceptibility of malignant melanoma
• Grantee: Cristiane de Oliveira
• Support Opportunities: Scholarships in Brazil - Master