Study of three axenic culture media for rust in Myrtaceae

Author(s)

Marilia Pizetta ^[1] ; Caroline Geraldi Pierozzi ^[2] ; Gleice Viviane Nunes Pereira ^[3] ; Juliana Cristina Sodário Cruz ^[4] ; Christiane Ceriani Aparecido ^[5] ; Martha Maria Passador ^[6] ; Edson Luiz Furtado ^[7]

Affiliation

^[1] UNESP. Faculdade de Ciências Agronômicas. Defesa Fitossanitária - Brasil

^[2] UNESP. Faculdade de Ciências Agronômicas. Defesa Fitossanitária - Brasil

^[3] UNESP. Faculdade de Ciências Agronômicas. Defesa Fitossanitária - Brasil

^[4] Agência Paulista de Tecnologia do Agronegócio - Brasil

^[5] Instituto Biológico. Centro de Pesquisas e Desenvolvimento de Sanidade Vegetal - Brasil

^[6] UNESP. Faculdade de Ciências Agronômicas. Defesa Fitossanitária - Brasil

^[7] UNESP. Faculdade de Ciências Agronômicas. Defesa Fitossanitária - Brasil

• Document type: Journal article
• Source: Summa Phytopathologica; v. 42, n. 2, p. 165-169, 2016-06-00.

Abstract

ABSTRACT Axenic cultivation has been shown as an important tool for studies related to the biology, the pathogenicity and the maintenance of pure cultures of biotrophic species. Therefore, the present study aimed to analyze the in vitro growth of Puccinia psidii uredospores, using enriched culture media that had success in the axenic cultivation of other rusts. Compounds such as salts, amino acids, carbohydrates and vitamins were selected for the preparation of three enriched culture media: ASZV Kuck (10), SH Schenk & Hildebrandt (21) and HG Harvey & Grasham (7), modified. Comparatively, enriched media were prepared from leaves of “jambeiro” (FJ) at four concentrations: 0.5; 1.0; 3.0 and 5.0 g L-1 (grams of leaves per one liter of distilled water). In this study, verification covered only the first stage of fungal growth, germ tube growth, not continuing in the pathogen development. The enriched media that provided greater P. psidii germ tube growth were the media ASZV, HG and FJ at 0.5 g L-1 concentration. (AU)

• FAPESP's process: 11/04423-8 - Evaluation of media culture for isolation and axenic culture of Puccinia psidii
• Grantee: Marília Pizetta
• Support Opportunities: Scholarships in Brazil - Master