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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus

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Leite, Carla A. [1] ; Cavallieri, Andre P. [1] ; Baptista, Amanda S. [1] ; Araujo, Maria L. G. C. [1]
Total Authors: 4
[1] UNESP Sao Paulo State Univ, Inst Chem, Dept Biochem & Technol Chem, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: FEMS Microbiology Letters; v. 363, n. 1 JAN 2016.
Web of Science Citations: 1

Streptomyces clavuligerus produces simultaneously cephamycin C (CephC) and clavulanic acid (CA). Adding 1,3-diaminopropane to culture medium stimulates production of beta-lactam antibiotics. However, there are no studies on the influence of this diamine on coordinated production of CephC and CA. This study indicates that 1,3-diaminopropane can dissociate CephC and CA productions. Results indicated that low diamine concentrations (below 1.25 g l(-1)) in culture medium increased CA production by 200%, but not that of CephC. Conversely, CephC production increased by 300% when 10 g l(-1) 1,3-diaminopropane was added to culture medium. Addition of just L-lysine (18.3 g l(-1)) to culture medium increased both biocompounds. On the other hand, while L-lysine plus 7.5 g l(-1) 1,3-diaminopropane increased volumetric production of CephC by 1100%, its impact on CA production was insignificant. The combined results suggest that extracellular concentration of 1,3-diaminopropane may trigger the dissociation of CephC and CA biosynthesis in S. clavuligerus. (AU)

FAPESP's process: 12/22181-4 - Monitoring of submerged cultures of Streptomyces clavuligerus strains for metabolic flux distribution analysis
Grantee:Amanda Salvador Baptista
Support type: Scholarships in Brazil - Master
FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
Grantee:Carlos Osamu Hokka
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/02632-4 - Metabolic flux analysis for the in silico optimization of the production of biocompounds by Streptomyces clavuligerus
Grantee:Maria Lucia Gonsales da Costa Araujo
Support type: Regular Research Grants