CHARACTERIZATION OF MICROSATELLITE MARKERS FOR BACCHARIS DRACUNCULIFOLIA (ASTERACEAE)

Author(s)

Belini, Camila M. B. ^[1] ; Marques, Marcia O. M. ^[2] ; Figueira, Glyn M. ^[3] ; Bajay, Miklos M. ^[4] ; Campos, Jaqueline B. ^[5] ; Viana, Joao P. G. ^[5] ; Pinheiro, Jose B. ^[4] ; Zucchi, Maria I. ^[6]

Affiliation

^[1] Univ Estadual Paulista, Programa Posgrad Hort, Rua Jose Barbosa de Barros 1780, BR-18610307 Botucatu, SP - Brazil

^[2] Inst Agron Campinas, Dept Fitoquim, Ave Doutor Theodureto Almeida Camargo 1500, BR-13012970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, CP 6171, Rua Alexandre Cazelatto 999, BR-13081970 Paulinia, SP - Brazil

^[4] Univ Sao Paulo, Dept Genet, Av Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[5] Univ Estadual Campinas, Programa Posgrad Genet & Biol Mol, CP 6109, Rua Bertrand Russel, BR-13083970 Campinas, SP - Brazil

^[6] Agencia Paulista Tecnol Agronegocios, Polo Ctr Sul, CP 28, Rodovia SP 127, Km 30, BR-13400970 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: APPLICATIONS IN PLANT SCIENCES; v. 4, n. 3 MAR 2016.
• Web of Science Citations: 0

Abstract

Premise of the study: Baccharis dracunculifolia (Asteraceae) is a native plant of the Atlantic Forest that is used for the production of essential oil. Microsatellite markers were developed for this species to investigate the genetic diversity of three natural populations. Methods and Results: Seventeen out of 27 microsatellite loci identified in a genomic library used for the characterization of 315 individuals derived from three natural populations of B. dracunculifolia resulted in successful amplifications. Eleven polymorphic loci, ranging from two to seven alleles per locus, were obtained with expected and observed heterozygosity values ranging between 0.068 and 0.775 and 0.046 and 0.667, respectively. Conclusions: The microsatellite loci described in this study are tools that can be used for further studies of population genetics of B. dracunculifolia with a focus on deforested areas and conservation of natural populations. (AU)

• FAPESP's process: 12/14136-9 - Genetic and chemical diversity of natural populations and evaluation of productivity in provenances and progenies test of Baccharis dracunculifolia D.C. (Asteraceae)
• Grantee: Glyn Mara Figueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/05052-9 - CONSERVATION AND EVALUATION OF ESSENTIAL OIL COMPOSITION AND GENETIC DIVERSITY OF NATURAL POPULATIONS AND EVALUATION OF PRODUCTIVITY IN PROVENANCES AND PROGENIES TEST OF Baccharis dracunculifolia DC. (Asteraceae)
• Grantee: Camila Moreira Batista Belini
• Support Opportunities: Scholarships in Brazil - Doctorate