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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A case study in flux balance analysis: Lysine, a cephamycin C precursor, can also increase clavulanic acid production

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Cavallieri, Andre Pastrelo [1] ; Baptista, Amanda Salvador [1] ; Leite, Carla Andrea [1] ; Gonsales da Costa Araujo, Maria Lucia [1]
Total Authors: 4
[1] UNESP Sso Paulo State Univ, Inst Chem, Dept Biochem & Technol Chem, Prof Francisco Degni St 55, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Biochemical Engineering Journal; v. 112, p. 42-53, AUG 15 2016.
Web of Science Citations: 2

Streptomyces clavuligerus is an excellent model for studying complex relationships involved in the biosynthesis of secondary metabolites. Despite the fact that several studies have investigated the mechanisms pertaining to the biosynthesis of two bioactive compounds produced by S. clavuligerus, cephamycin C (CephC) and clavulanic acid (CA), few have focused on metabolic flux analyses of this microorganism. It was proposed a novel representative metabolic model for S. clavuligerus addressing the simultaneous production of CephC and CA using flux balance analysis methodology (FBA). Although both are synthesized via different pathway, flux analysis showed that the production of these bioactives are connected. CephC production showed high dependency on N metabolism while CA production is more related with C metabolism. Adding lysine plus maltose increased CA, an interesting outcome since lysine is a CephC precursor. Notwithstanding, it was possible to propose a mechanism for this behavior by means of flux analysis. Furthermore, the results suggest that ATP yield maximization is the objective function that best suits the conditions under investigation. (c) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/22181-4 - Monitoring of submerged cultures of Streptomyces clavuligerus strains for metabolic flux distribution analysis
Grantee:Amanda Salvador Baptista
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/02632-4 - Metabolic flux analysis for the in silico optimization of the production of biocompounds by Streptomyces clavuligerus
Grantee:Maria Lucia Gonsales da Costa Araujo
Support type: Regular Research Grants