Cloxacillin control of experimental arthritis induced by SEC+ Staphylococcus aureus is associated with downmodulation of local and systemic cytokines

Author(s):Show less -

Colavite, Priscila Maria ^[1] ; Watanabe Ishikawa, Larissa Lumi ^[1] ; Goncalves Zorzella-Pezavento, Sofia Fernanda ^[1] ; Cardoso de Oliveira, Larissa Ragozo ^[1] ; Donega Franca, Thais Graziela ^[1] ; da Rosa, Larissa Camargo ^[1] ; Chiuso-Minicucci, Fernanda ^[1] ; Vieira, Andreia Espindola ^[2] ; Francisconi, Carolina Favaro ^[2] ; Ribeiro de Souza da Cunha, Maria de Lourdes ^[1] ; Garlet, Gustavo Pompermaier ^[2] ; Sartori, Alexandrina ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Sao Paulo - Brazil

^[2] Sao Paulo Univ FOB USP, Sch Dent Bauru, Dept Biol Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: Cellular Microbiology; v. 18, n. 7, p. 998-1008, JUL 2016.
• Web of Science Citations: 0

Abstract

Staphylococcus aureus is the most common agent of septic arthritis (SA) that is a severe, rapidly progressive and erosive disease. In this work we investigated the clinical, histopathological and immunological characteristics of the SA triggered by an enterotoxin C producer S. aureus strain. The effect of a -lactamic antibiotic over disease evolution and cytokine production was also evaluated. After confirmation that ATCC 19095 SEC+ strain preserved its ability to produce enterotoxin C, this bacteria was used to infect C57BL/6 male mice. Body weight, clinical score and disease prevalence were daily evaluated during 14days. Cytokine production by splenocytes, cytokine mRNA expression in arthritic lesions, transcription factors mRNA expression in inguinal lymph nodes and histopathological analysis were performed 7 and 14days after infection. ATCC 19095 SEC+ strain caused a severe arthritis characterized by weight loss, high clinical scores and a 100% disease prevalence. Histopathological analysis revealed inflammation, pannus formation and bone erosion. Arthritis aggravation was associated with elevated production of pro-inflammatory cytokines, higher local mRNA expression of these cytokines and also higher mRNA expression of T-bet, ROR- and GATA-3. Disease control by cloxacillin was associated with decreased production of pro-inflammatory cytokines but not of IL-10. These findings indicate that the ATCC 19095 SEC+ strain is able to initiate a severe septic arthritis in mice associated with elevated cytokine production that can be, however, controlled by cloxacillin. (AU)

• FAPESP's process: 11/04323-3 - Impact of the use of an immunomodulatory drug in the treatment of septic arthritis induced by Staphylococcus aureus
• Grantee: Priscila Maria Colavite Machado
• Support Opportunities: Scholarships in Brazil - Master