Identification of Candida haemulonii Complex Species: Use of ClinProTools (TM) to Overcome Limitations of the Bruker Biotyper (TM), VITEK MS (TM) IVD, and VITEK MS (TM) RUO Databases

Author(s):Show less -

Grenfell, Rafaella C. ^[1] ; da Silva Junior, Afonso R. ^[2] ; Del Negro, Gilda M. B. ^{[3, 4]} ; Munhoz, Regina B. ^[2] ; Gimenes, Viviane M. F. ^{[3, 4]} ; Assis, Diego M. ^[5] ; Rockstroh, Anna C. ^[6] ; Motta, Adriana L. ^[2] ; Rossi, Flavia ^[2] ; Juliano, Luiz ^[1] ; Benard, Gil ^{[3, 4]} ; de Almeida Junior, Joao N. ^{[3, 2, 4]}

Affiliation

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biophys, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Cent Lab Div LIM 03, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Hosp Clin FMUSP, Lab Med Mycol LIM 53, Sao Paulo - Brazil

^[5] Bruker Brasil, Atibaia - Brazil

^[6] bioMerieux, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN MICROBIOLOGY; v. 7, JUN 16 2016.
• Web of Science Citations: 14

Abstract

Candida haemulonli is now considered a complex of two species and one variety: C. haemulonii sensu strict, Candida duobushaemulonii and the variety C. haemulonii var. vulnera. Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species ID for these emergent pathogens. Hence, we evaluated the MALDI-TOF MS performance for the ID of C. haemulonli species, analyzing isolates/strains of C. haemulonli complex species, Candida pseudohaemulonii and Candida auris by two commercial platforms, their databases and softwares. To differentiate C. haemulonli sensu sctricto from the variety vulnera, we used the ClinProTools (TM) models and a single-peak analysis with the software FlexAnalysis (TM). The Biotyper (TM) database gave 100% correct species ID for C. haemulonii sensu strict, C. pseudohaemulonii and C. auris, with 69% of correct species ID for C. duobushaemulonii. Vitek MS (TM) IVD database gave 100% correct species ID for C. haemulonii sensu stricto, misidentifying all C. duobushaemulonii and C. pseudohaemulonii as C. haemulonii, being unable to identify C. auris. The Vitek MS (TM) RUO database needed to be upgraded with in-house SuperSpectra to discriminate C. haemulonii sensu stricto, C. duobushaemulonii, C. pseudohaemulonii, and C. auris strains/isolates. The generic algorithm model from ClinProTools (TM) software showed recognition capability of 100% and cross validation of 98.02% for the discrimination of C. haemulonli sensu stricto from the variety vulnera. Single-peak analysis showed that the peaks 5670, 6878, or 13750 m/z can distinguish C. haemulonli sensu stricto from the variety vulnera. (AU)

• FAPESP's process: 14/10126-4 - Clinical and microbiological features of Candida haemulonii complex isolates from HC-FMUSP
• Grantee: João Nóbrega de Almeida Júnior
• Support Opportunities: Regular Research Grants