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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation of the Pathogen Rickettsia sp Strain Atlantic Rainforest From Its Presumed Tick Vector, Amblyomma ovale (Acari: Ixodidae), From Two Areas of Brazil

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Author(s):
Nieri-Bastos, Fernanda A. [1] ; Horta, Mauricio C. [2] ; Barros-Battesti, Darci Moraes [3] ; Moraes-Filho, Jonas [1, 4] ; Ramirez, Diego G. [3] ; Martins, Thiago F. [1] ; Labruna, Marcelo B. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Vet Med, Dept Prevent Vet Med & Anim Hlth, Sao Paulo, SP - Brazil
[2] Fed Univ Vale do Sao Francisco, Parasit Dis Lab, Petrolina, Pernambuco - Brazil
[3] Butantan Inst, Parasitol Lab, Sao Paulo, SP - Brazil
[4] Santo Amaro Univ UNISA, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Medical Entomology; v. 53, n. 4, p. 977-981, JUL 2016.
Web of Science Citations: 8
Abstract

In this study, Amblyomma ovale Koch ticks were collected from domestic dogs in two localities of the Atlantic rainforest biome of Brazil: 1) the Paty Valley of the Chapada Diamantina National Park, Bahia state (northeastern Brazil), and 2) Adrianopolis, Parana state (southern Brazil). Ticks were screened for the presence of Rickettsia-like structures by the hemolymph test with Gimenez staining, and then processed for isolation of rickettsiae in Vero cell culture by the shell-vial technique. Rickettsiae were isolated from one A. ovale tick of each of the two localities. The two isolates were successfully established in the laboratory with several passages, each one reaching >90% infection of the cells. The two isolates were identified as the spotted fever group (SFG) agent Rickettsia sp. strain Atlantic rainforest, as their gltA (350 bp), ompB (781 bp), and ompA (567 bp) gene fragments were 100% equal to GenBank corresponding sequences of the original strain Atlantic rainforest, reported to be infecting a human in southeastern Brazil, and also 100% equal to the available ompA sequence of strain Bahia, reported to be infecting a human in Paty Valley, the same area of the present study in Bahia state. Ten dogs from Paty Valley were serologically tested against rickettsial antigens by the indirect immunofluorescence antibody test. At least 60% of them were seroreactive to SFG rickettsiae. The role of A. ovale as vector of Rickettsia sp. strain Atlantic rainforest in the Paty Valley area, as well as in other parts of Latin America, is discussed. (AU)

FAPESP's process: 11/51979-1 - Molecular characterization and multigenic phylogeography of Rickettsia parkeri isolates in the Americas
Grantee:Fernanda Aparecida Nieri Bastos
Support Opportunities: Scholarships in Brazil - Post-Doctoral