| Full text | |
| Author(s): |
Carvalho, F. M. C.
[1, 2]
;
Busser, F. D.
[2]
;
Freitas, V. L. T.
[2]
;
Furucho, C. R.
[2]
;
Sadahiro, A.
[3]
;
Kono, A. S. G.
[4]
;
Criado, P. R.
[5]
;
Moretti, M. L.
[6]
;
Sato, P. K.
[1, 2]
;
Shikanai-Yasuda, M. A.
[1, 2]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Av Dr Eneas Carvalho Aguiar 470 IMT1, 1 Andar, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Med Invest Immunol LIM 48, Av Dr Eneas Carvalho Aguiar 470 IMT2, Sala 12, BR-05403000 Sao Paulo, SP - Brazil
[3] Univ Fed Amazonas, Inst Ciencias Biol, Dept Parasitol, Av Gen Rodrigo Octavio 6200, BR-69077000 Coroado, Amazonas - Brazil
[4] Univ Sao Paulo, Hosp Clin, Div Infect Dis, Av Dr Eneas Carvalho Aguiar 155, 5 Andar, Bloco 2A, BR-05403000 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Hosp Clin, Dermatol Clin Div, Av Dr Eneas Carvalho Aguiar 255, 5 Andar, Bloco 2B, BR-05403000 Sao Paulo, SP - Brazil
[6] Univ Estadual Campinas, Fac Ciencias Med, Hosp Clin, Rua Vital Brasil 251, Cidade Univ, BR-13083888 Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | INFECTION GENETICS AND EVOLUTION; v. 43, p. 245-251, SEP 2016. |
| Web of Science Citations: | 7 |
| Abstract | |
Paracoccidioidomycosis (PCM) is a systemic chronic mycosis, endemic in Latin America, especially Brazil, and is the eighth leading cause of death among chronic and recurrent infectious diseases. PCM infection is characterized by the presence of Th1 immune response; the acute form, by a mixed Th2/Th9, while the chronic form is characterized by Th17/Th22 profiles. The occurrence and severity of human PCM may also be associated with genetic factors such as single nucleotide polymorphisms (SNP) on cytokines encoding genes. We investigated the association between these polymorphisms and the different clinical forms of PCM. We included 156 patients with PCM(40 with the acute form, 99 with the chronic multifocal and 17 with the chronic unifocal form) and assayed their DNA samples for IFNG + 874 T/A SNP by PCR-ARMS (Amplification Refractory Mutational System), IL12B + 1188 A/C SNP on 3' UTR and IL12RB1 641 A/G SNP on exon 7 by PCR-RFLP (Restriction Fragment Length Polymorphism). We found similar genotypic and allelic frequencies of the investigated SNPs among the clinical forms of PCM. Considering male patients, the IL12RB1 641 AA genotype was more frequent in the chronic multifocal form while heterozygosis was in the chronic unifocal form of PCM (p = 0.048). Although our data suggest that the AA genotype (IL12RB1) may be associated with the more disseminated chronic disease, more patients of the chronic unifocal PCM group need to be analyzed as well as the secretion patterns of IFN-gamma combined with the IL-12R beta 1 expression for a better comprehension of this association. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/25192-7 - Human paracoccidioidomycosis: association study between cytokines genetic polymorphisms and immune responses on different clinical presentations of the disease |
| Grantee: | Maria Aparecida Shikanai Yasuda |
| Support Opportunities: | Regular Research Grants |