Renal Subcapsular Space of Balb/c Nude Mice as a Route for Evaluating Subpopulations of Human Bladder Carcinoma Cells

Author(s)

Souza, Diego Mota ^[1] ; Gomes Matheus, Luiz Henrique ^[1] ; Silva, Chrisna Souza ^[1] ; Ferreira, Janaina Mendes ^[1] ; Delle, Humberto ^[1]

Affiliation

^[1] Nove de Julho Univ UNINOVE, Postgrad Program Med, Rua Vergueiro 235, 2 Subsolo, BR-01504001 Sao Paulo - Brazil

• Document type: Review article
• Source: IN VIVO; v. 30, n. 4, p. 383-386, JUL-AUG 2016.
• Web of Science Citations: 0

Abstract

Background/Aim: Subpopulations of bladder cancer (BC) cells have been found in tumors, with different abilities for malignancy and chemotherapy resistance. The BC cell line T24 has frequently been used to evaluate this phenomenon. Since technical limits exist in orthotopic procedures, we evaluated the renal subcapsular space as an alternative route for analyzing subpopulations of T24 BC cells in vivo. Materials and Methods: Balb/c nude mice underwent renal subcapsular inoculation with T24 cells, suspended in two different volumes of PBS. Four weeks post-inoculation, histology and immunohistochemistry were carried out. Results: In all the animals inoculated with a 10 mu l volume of suspended cells, a pseudo-bladder structure in the renal subcapsular space was observed, with differential expression of mesenchymal and epithelial markers. T24 cells infiltrating the renal parenchyma towards the medulla and vessels were also observed. The volume used for inoculation was an important factor for the success of this technique. Conclusion: Renal subcapsular inoculation is an effective route for analyzing subpopulations and differentiation of T24 cells. (AU)

• FAPESP's process: 12/04423-0 - Role of indoleamine 2,3 dioxygenase (IDO) in the process of epithelial-mesenchymal transition(EMT) mediated by TGF-beta in human urogenital carcinoma
• Grantee: Humberto Dellê
• Support Opportunities: Regular Research Grants