Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

Full text
Author(s):
Verônica Chomiski [1] ; Alfredo Gragnani [2] ; Jéssica Bonucci [3] ; Silvana Aparecida Alves Correa [4] ; Samuel Marcos Ribeiro de Noronha [5] ; Lydia Masako Ferreira [6]
Total Authors: 6
Affiliation:
[1] Universidade Federal de São Paulo. Department of Surgery. Division of Plastic Surgery - Brasil
[2] UNIFESP. Department of Surgery. Division of Plastic Surgery - Brasil
[3] UNIFESP. Department of Surgery. Division of Plastic Surgery - Brasil
[4] UNIFESP. Department of Surgery. Division of Plastic Surgery - Brasil
[5] UNIFESP. Department of Surgery. Division of Plastic Surgery - Brasil
[6] UNIFESP. Division of Plastic Surgery - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Acta Cirurgica Brasileira; v. 31, n. 8, p. 505-512, 2016-08-00.
Abstract

ABSTRACT PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor. (AU)

FAPESP's process: 13/10905-0 - Keratinocyte growth factor in gene expression of immune system and wound healing in stem cells of the skin from burned patients
Grantee:Alfredo Gragnani Filho
Support Opportunities: Regular Research Grants