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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of two novel leptospiral proteins for their interaction with human host components

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Silva, Lucas P. ; Fernandes, Luis G. V. ; Vieira, Monica L. ; de Souza, Gisele O. ; Heinemann, Marcos B. ; Vasconcellos, Silvio A. ; Romero, Eliete C. ; Nascimento, Ana L. T. O.
Total Authors: 8
Document type: Journal article
Source: PATHOGENS AND DISEASE; v. 74, n. 5 JUL 2016.
Web of Science Citations: 10

Pathogenic species of the genus Leptospira are the etiological agents of leptospirosis, the most widespread zoonosis. Mechanisms involved in leptospiral pathogenesis are not well understood. By data mining the genome sequences of Leptospira interrogans we have identified two proteins predicted to be surface exposed, LIC10821 and LIC10064. Immunofluorescence and proteinase K assays confirmed that the proteins are exposed. Reactivity of the recombinant proteins with human sera has shown that rLIC10821, but not rLIC10064, is recognized by antibodies in confirmed leptospirosis serum samples, suggesting its expression during infection. The rLIC10821 was able to bind laminin, in a dose-dependent fashion, and was called Lsa37 (leptospiral surface adhesin of 37 kDa). Studies with human plasma components demonstrated that rLIC10821 interacts with plasminogen (PLG) and fibrinogen (Fg). The binding of Lsa37 with PLG generates plasmin when PLG activator was added. Fibrin clotting reduction was observed in a thrombin-catalyzed reaction, when Fg was incubated with Lsa37, suggesting that this protein may interfere in the coagulation cascade during the disease. Although LIC10064 protein is more abundant than the corresponding Lsa37, binding activity with all the components tested was not detected. Thus, Lsa37 is a novel versatile adhesin that may mediate Leptospira-host interactions. (AU)

FAPESP's process: 12/24164-0 - Characterization of the interaction of Leptospira interrogans with prothrombin/thrombin system and possible implications in virulence
Grantee:Luis Guilherme Virgílio Fernandes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/50981-0 - Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis
Grantee:Ana Lucia Tabet Oller Do Nascimento
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/23913-9 - Indentification and characterization of proteins of Leptospira interrogans involved in host-pathogen interactions
Grantee:Ana Lucia Tabet Oller Do Nascimento
Support type: Regular Research Grants
FAPESP's process: 14/18337-4 - Modulação da coagulação e inflamação do hospedeiro por Leptospira
Grantee:Mônica Larucci Vieira
Support type: Scholarships abroad - Research Internship - Post-doctor