Maternal vitamin B-6 deficient or supplemented diets on expression of genes related to GABAergic, serotonergic, or glutamatergic pathways in hippocampus of rat dams and their offspring

Author(s)

Almeida, Mara Ribeiro ; Mabasa, Lawrence ; Crane, Courtney ; Park, Chung S. ; Venancio, Vinicius Paula ; Pires Bianchi, Maria Lourdes ; Greggi Antunes, Lusania Maria

• Document type: Journal article
• Source: MOLECULAR NUTRITION & FOOD RESEARCH; v. 60, n. 7, p. 1615-1624, JUL 2016.
• Web of Science Citations: 2

Abstract

ScopeVitamin B-6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at evaluating which components of those neurotransmitter metabolism and signaling pathways can be modulated by maternal vitamin B-6-deficient or B-6-supplementated diets in the hippocampus of rat dams and their offspring. Methods and resultsFemale Wistar rats were fed three different diets: control (6 mg vitamin B-6/kg), supplemented (30 mg vitamin B-6/kg) or deficient diet (0 mg vitamin B-6/kg), from 4 weeks before pregnancy through lactation. Newborn pups (10 days old) from rat dams fed vitamin B-6-deficient diet presented hyperhomocysteinemia and had a significant increase in mRNA levels of glutamate decarboxylase 1 (Gad1), fibroblast growth factor 2 (Fgf2), and glutamate-ammonia ligase (Glul), while glutaminase (Gls) and tryptophan hydroxylase 1 (Tph1) mRNAs were downregulated. Vitamin B-6 supplementation or deficiency did not change hippocampal global DNA methylation. ConclusionA maternal vitamin B-6-deficient diet affects the expression of genes related to GABA, glutamate, and serotonin metabolisms in offspring by regulating Gad1, Glul, Gls, and Tph1 mRNA expression. (AU)

• FAPESP's process: 12/20441-9 - Evaluation of the chromosomal instability and mRNA expression of genes related to neurogenesis and neurotransmitter metabolism in the offspring from dams treated with vitamin B6 deficient or supplemented diets
• Grantee: Mara Ribeiro de Almeida
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/01288-0 - Effect of maternal dietary vitamin B6 on the modulation of gene expression in brain of rat dams and their offspring
• Grantee: Mara Ribeiro de Almeida
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor