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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Renin-angiotensin system blockers regulate the metabolism of isolated fat cells in vitro

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Author(s):
Caminhotto, R. de O. ; Sertie, R. A. L. ; Andreotti, S. ; Campana, A. B. ; Lima, F. B.
Total Authors: 5
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 49, n. 8 2016.
Web of Science Citations: 1
Abstract

Due to the presence of the renin-angiotensin system (RAS) in tissues and its specific influence on white adipose tissue, fat cells are possible targets of pharmacological RAS blockers commonly used as anti-hypertensive drugs. In the present study, we investigated the effects of different RAS blockers on fat cell metabolism, more specifically on lipolysis, lipogenesis and oxidation of energy substrates. Isolated primary adipocytes were incubated with different RAS blockers (aliskiren, captopril and losartan) in vitro for 24 h and lipolysis, lipogenesis and glucose oxidation capacities were determined in dose-response assays to a β-adrenergic agonist and to insulin. Although no change was found in lipolytic capacity, the RAS blockers modulated lipogenesis and glucose oxidation in a different way. While captopril decreased insulin-stimulated lipogenesis (−19% of maximal response and −60% of insulin responsiveness) due to reduced glucose derived glycerol synthesis (−19% of maximal response and 64% of insulin responsiveness), aliskiren increased insulin-stimulated glucose oxidation (+49% of maximal response and +292% of insulin responsiveness) in fat cells. Our experiments demonstrate that RAS blockers can differentially induce metabolic alterations in adipocyte metabolism, characterized by a reduction in lipogenic responsiveness or an increase in glucose oxidation. The impact of RAS blockers on adipocyte metabolism may have beneficial implications on metabolic disorders during their therapeutic use in hypertensive patients. (AU)

FAPESP's process: 12/02054-8 - Pharmacological blockade of the renin-angiotensin system and the regulation of metabolism in isolated fat cells
Grantee:Rennan de Oliveira Caminhotto
Support Opportunities: Scholarships in Brazil - Master