| Full text | |
| Author(s): |
Goncalves, Heloisa Bressan
;
Jorge, Joao Atilio
;
Souza Guimaraes, Luis Henrique
Total Authors: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF FOOD BIOCHEMISTRY; v. 40, n. 5, p. 655-663, OCT 2016. |
| Web of Science Citations: | 3 |
| Abstract | |
The search for new sources of beta-D-fructofuranosidases with potential for utilization in the food and beverage industries is an important task. The filamentous fungus Fusarium graminearum was recently reported to produce beta-D-fructofuranosidase with suitable properties for biotechnological applications. Therefore, the objective of this study was to purify and characterize F. graminearum beta-D-fructofuranosidase. High levels of the enzyme were obtained in Solid-State Fermentation (at 30C for 7 days) using wheat bran as a carbon source. The extracellular enzyme was purified 8-fold with 14% recovery using ethanol precipitation, diethylaminoethyl-Cellulose, and Sephacryl S-200. The optimum temperature and pH for the heterodimeric protein (94 kDa and 66 kDa), were 55-60C and 4.5, respectively. The enzyme was stable at 30-50C for 1 h, and at pH 3.0-8.0. Enzymatic activity was enhanced by Mn2+ (127%) and was inhibited by Hg2+. The Km values were 31.6 and 24.1 mM for sucrose and raffinose, respectively. PRACTICAL APPLICATIONS beta-D-Fructofuranosidases are enzymes with a wide range of industrial applications, especially in the food and beverage industries. These enzymes catalyze the hydrolysis of sucrose to invert sugar syrup. In addition, some beta-D-fructofuranosidases can catalyze transfructosylation reaction for production of fructooligosaccharides (FOSes). Both invert sugar and FOSes are important materials for the food industry. The main sources of beta-D-fructofuranosidase are microorganisms; the filamentous fungus Fusarium graminearum is a new source of beta-D-fructofuranosidase with attractive properties for practical applications. The characterization of F. graminearum b-D-fructofuranosidase is an important step to determine its potential practical applications. (AU) | |
| FAPESP's process: | 09/01283-0 - Characterization of fructo-oligosacharides and b-fructosidases with biotechnological interest produced by filamentous fungi |
| Grantee: | Luis Henrique Souza Guimaraes |
| Support Opportunities: | Regular Research Grants |