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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analgesic Effect of Photobiomodulationon Bothrops Moojeni Venom-Induced Hyperalgesia: A Mechanism Dependent on Neuronal Inhibition, Cytokines and Kinin Receptors Modulation

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Author(s):
Nadur-Andrade, Nikele ; Dale, Camila Squarzoni ; da Silva Oliveira, Victoria Regina ; Toniolo, Elaine Flamia ; Feliciano, Regiane dos Santos ; da Silva, Jr., Jose Antonio ; Zamuner, Stella Regina
Total Authors: 7
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 10, n. 10 OCT 2016.
Web of Science Citations: 6
Abstract

Background Envenoming induced by Bothrops snakebites is characterized by drastic local tissue damage that involves an intense inflammatory reaction and local hyperalgesia which are not neutralized by conventional antivenom treatment. Herein, the effectiveness of photobiomodulation to reduce inflammatory hyperalgesia induced by Bothrops moojeni venom (Bmv), as well as the mechanisms involved was investigated. Methodology/Principal Findings Bmv (1 mu g) was injected through the intraplantar route in the right hind paw of mice. Mechanical hyperalgesia and allodynia were evaluated by von Frey filaments at different time points after venom injection. Low level laser therapy (LLLT) was applied at the site of Bmv injection at wavelength of red 685 nm with energy density of 2.2 J/cm(2) at 30 min and 3 h after venom inoculation. Neuronal activation in the dorsal horn spinal cord was determined by immunohistochemistry of Fos protein and the mRNA expression of IL-6, TNF-alpha, IL-10, B1 and B2 kinin receptors were evaluated by Real time-PCR 6 h after venom injection. Photobiomodulation reversed Bmv-induced mechanical hyperalgesia and allodynia and decreased Fos expression, induced by Bmv as well as the mRNA levels of IL-6, TNF-a and B1 and B2 kinin receptors. Finally, an increase on IL-10, was observed following LLLT. Conclusion/Significance These data demonstrate that LLLT interferes with mechanisms involved in nociception and hyperalgesia and modulates Bmv-induced nociceptive signal. The use of photobiomodulation in reducing local pain induced by Bothropic venoms should be considered as a novel therapeutic tool for the treatment of local symptoms induced after bothropic snakebites. (AU)

FAPESP's process: 12/09710-8 - Effect of photobiostimulation on the local effect induced by Bothrops moojeni and myotoxins isolated.
Grantee:Nikele Nadur Andrade
Support Opportunities: Scholarships in Brazil - Doctorate