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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Subchronic administration of phencyclidine produces hypermethylation in the parvalbumin gene promoter in rat brain

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Author(s):
Fachim, Helene A. ; Srisawat, Umarat ; Dalton, Caroline F. ; Harte, Michael K. ; Marsh, Samuel ; Neill, Joanna C. ; Reynolds, Gavin P.
Total Authors: 7
Document type: Journal article
Source: Epigenomics; v. 8, n. 9, p. 1179-1183, SEP 2016.
Web of Science Citations: 8
Abstract

Aim: A deficit in parvalbumin neurons is found in schizophrenia and several animal models of the disease. In this preliminary study, we determined whether one such model, phencyclidine (PCP) administration, results in changes in DNA methylation in the rat Pvalb promoter. Materials \& methods: DNA from hippocampus and prefrontal cortex from rats, which 6 weeks previously received either 2 mg/kg PCP or vehicle for 7 days, underwent bisulphite pyrosequencing to determine methylation. Results: PCP administration induced significantly greater methylation at one of two Pvalb CpG sites in both prefrontal cortex and hippocampus, while no significant difference was found in long interspersed nucleotide element-1, a global measure of DNA methylation. Conclusion: Subchronic PCP administration results in a specific hypermethylation in the Pvalb promoter which may contribute to parvalbumin deficits in this animal model of psychosis. (AU)

FAPESP's process: 15/02948-7 - Study of epigenetic alterations in GABAergic neurotransmission in schizophrenia and other psychoses and in animals undergoing social isolation from weaning
Grantee:Helene Aparecida Fachim
Support Opportunities: Scholarships in Brazil - Post-Doctoral