Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantitative Proteomic Profiling Reveals That Diverse Metabolic Pathways Are Influenced by Melatonin in an in Vivo Model of Ovarian Carcinoma

Full text
Author(s):
Chuffa, Luiz Gustavo A. ; Lupi Junior, Luiz Antonio ; Seiva, Fabio R. F. ; Martinez, Marcelo ; Domeniconi, Raquel F. ; Pinheiro, Patricia Fernanda F. ; dos Santos, Lucilene D. ; Martinez, Francisco Eduardo
Total Authors: 8
Document type: Journal article
Source: JOURNAL OF PROTEOME RESEARCH; v. 15, n. 10, p. 3872-3882, OCT 2016.
Web of Science Citations: 14
Abstract

To obtain more information into the molecular mechanisms underlying ovarian cancer (OC), we proposed a comparative proteomic analysis in animals receiving long-term melatonin as therapy or only vehicle using multidimensional protein identification combined with mass spectrometry. To induce tumor, a single dose of 100 mu g 7,12-dimethylbenz(a)-anthracene (DMBA) dissolved in 10 mu L of sesame oil was injected under the left ovarian bursa of 20 Fischer 344 rats. The right ovaries were injected with sesame oil only. After tumors were developed, half of the animals received intraperitoneal administration of melatonin (200 mu g/100g body weight/day) for 60 days. Melatonin therapy promoted down-regulation in numerous proteins involved in OC signaling pathways. The most significant portion of these proteins are involved in several metabolic processes, mainly those associated with mitochondrial systems, generation of metabolites and energy, hypoxia-inducible factor-1 signaling, antigen processing and presentation, endoplasmic reticulum stress-associated pathways, and cancer-related proteoglycans. A small number of proteins that were overexpressed by melatonin therapy included ATP synthase subunit beta, fatty acid-binding protein, and 10-kDa heat shock protein. Taken together, our findings suggest that melatonin therapy efficiently modulated important signaling pathways involved in OC, and these proteins might be further targets that should be explored in new therapeutic opportunities for OC. (AU)

FAPESP's process: 13/02466-7 - Induction of ovarian tumor and influence of melatonin on inflammatory signaling pathway mediated by Toll-like receptor 4 in UChB rats
Grantee:Luiz Gustavo de Almeida Chuffa
Support Opportunities: Regular Research Grants