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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Merozoite-Protein Loaded Liposomes Protect against Challenge in Two Murine Models of Plasmodium Infection

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Author(s):
Fotoran, Wesley L. ; Colhone, Marcelle C. ; Ciancaglini, Pietro ; Stabeli, Rodrigo G. ; Wunderlich, Gerhard
Total Authors: 5
Document type: Journal article
Source: ACS BIOMATERIALS SCIENCE & ENGINEERING; v. 2, n. 12, p. 2276-2286, DEC 2016.
Web of Science Citations: 2
Abstract

Liposomes loaded with GPI-anchored proteins induce a strong and effective response against protozoan pathogens when immunized in mice. On the basis of results using Plasmodium falciparum merozoite proteins loaded on liposomes, we tested whether a similar approach would be protective in the P. yoelii XL and P. berghei NK65 models of lethal murine malaria infections. We also analyzed the enhancing capacity of additional adjuvants. As expected, merozoite protein loaded liposomes provided strong humoral responses (IgG titers of 10(5) against MSPl(19)) in BALB/c and CS7BL/6 host backgrounds and showed total protection in lethal challenges (100% survival) with P. yoelii, while protection against lethal challenge with P. berghei NK was at least partial (11%-20% in C57BL/6 mice and no protection in BALB/c). Thus, immunization with proteoliposomes is not only highly immunogenic but also provides a protective response in widely used murine models of malaria. (AU)

FAPESP's process: 12/23306-5 - Studies on the mechanism of transcriptional control and switching of virulence-associated genes in Plasmodium falciparum
Grantee:Gerhard Wunderlich
Support Opportunities: Regular Research Grants
FAPESP's process: 15/17174-7 - Factors influencing expression of variant antigen families var, rif and surf of Plasmodium falciparum, the human malaria parasite
Grantee:Gerhard Wunderlich
Support Opportunities: Regular Research Grants
FAPESP's process: 12/18617-1 - Role of variant antigens in the immunity against murine Plasmodium: Estimating the influence of variant proteins BIR in cytoadherence and their potential as vaccine using novel means of administration.
Grantee:Wesley Luzetti Fotoran
Support Opportunities: Scholarships in Brazil - Doctorate