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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative sperm ultrastructure of twelve leptodactylid frog species with insights into their phylogenetic relationships

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Author(s):
Santos, Julio Sergio ; Introini, Gisele Orlandi ; Prado Veiga-Menoncello, Ana Cristina ; Blasc, Ailin ; Rivera, Miryan ; Recco-Pimentel, Shirlei Maria
Total Authors: 6
Document type: Journal article
Source: Micron; v. 91, p. 1-10, DEC 2016.
Web of Science Citations: 1
Abstract

The spermatozoa of representatives of three Neotropical frog subfamilies, Leiuperinae, Leptodactylinae and Paratelmatobiinae, were observed using Transmission Electron Microscopy, with the aim of identifying ultrastructural traits that provide insights into the phylogenetic relationships among these anurans, which are currently unclear. In the leiuperines, spermatozoa of Physalaemus albifrons, P. cicada, P. deimaticus and P. feioi were characterized by an acrosomal vesicle covering the subacrosomal cone that was not observed in the spermatozoa of Physalaemus centralis and P. cuvieri. The tail of the spermatozoa of P. albifrons, P. centralis, P. cicada, P. cuvieri, P. deimaticus, and P. feioi presented a long undulating membrane, whereas Engystomops petersi and E. freibergi, which form a sister Glade to Physalaemus, had an axial fiber, which were absent in Physalaemus. Other leiuperine, E. puyango had an abaxonemal bulb-like swelling distally to the paraxonemal rod, which were also absent in Physalaemus. These differences support the revalidation of Engystomops as a true taxon, distinct from Physalaemus. The tail of the spermatozoa of E. petersi and E. freibergi was similar to that of Paratelmatobius poecilogaster (Paratelmatobiinae). The spermatozoa of Leptodactylus natalenis (Leptodactylinae) had undulating membrane and axial fiber, in contrast with Adenomera marmorata, which lacked these structures. Morphological differences between A. marmorata and L natalensis sperm cells appeared to validate the allocation of A. marmorata into a genus distinct from Leptodactylus. Overall, dissimilarities in the spermatozoa of the leptodactylids provided an important phylogenetic signal for the understanding of their taxonomic relationships. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/04076-1 - Comparative study of sperm ultrastructure of frog species of the subfamily Leiuperinae (Amphibia, Anura, Leptodactylidae)
Grantee:Shirlei Maria Recco-Pimentel
Support type: Regular Research Grants