| Full text | |
| Author(s): |
Arena, Gabriella D.
;
Ramos-Gonzalez, Pedro L.
;
Nunes, Maria A.
;
Ribeiro-Alves, Marcelo
;
Camargo, Luis E. A.
;
Kitajima, Elliot W.
;
Machado, Marcos A.
;
Freitas-Astua, Juliana
Total Authors: 8
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PLANT SCIENCE; v. 7, NOV 25 2016. |
| Web of Science Citations: | 10 |
| Abstract | |
Leprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERE branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferous ones. Oviposition of the viruliferous mites occurred preferentially in the CiLV-C infected leaves. Based on these results, we postulated the first model of plant/Brevipalpus mite/cilevirus interaction in which cells surrounding the feeding sites of viruliferous mites typify the outcome of a hypersensitive-like response, whereas viral infection induces changes in the behavior of its vector. (AU) | |
| FAPESP's process: | 12/18771-0 - Gene expression analysis of model plant in response to citrus leprosis |
| Grantee: | Gabriella Dias Arena |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite |
| Grantee: | Elliot Watanabe Kitajima |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 08/57909-2 - Genomic platforms applied to citrus breeding |
| Grantee: | Marcos Antonio Machado |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/00366-8 - Arabidopsis thaliana e Citrus leprosis virus-C: a model for the plant-pathogen interaction study |
| Grantee: | Gabriella Dias Arena |
| Support Opportunities: | Scholarships in Brazil - Doctorate |