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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fermentative production of H-2 from different concentrations of galactose by the new isolate Clostridium beijerinckii Br21

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Author(s):
Fonseca, Bruna Constante ; Guazzaroni, Maria-Eugenia ; Reginatto, Valeria
Total Authors: 3
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 41, n. 46, p. 21109-21120, DEC 14 2016.
Web of Science Citations: 7
Abstract

Algal biomass is a promising raw material for production of third-generation biohydrogen. Few studies have investigated how the concentration of galactose, the main monosaccharide of algae biomass, affects fermentative production of H-2. We isolated a new culture of H-2-producing bacterium from an anaerobic sludge. This culture was identified as Clostridium beijerinckii based on the sequence of the 16S rRNA gene and was named C. beijerinckii Br21 (KT626859). The isolated culture produced the highest amount of H-2 at 35 degrees C and initial pH 7. The isolate used many carbon sources to produce H-2, including the monosaccharides glucose, galactose, and mannose resulting from algae biomass hydrolysis. For the first time, fermentation batch tests based on galactose as carbon source showed that production of H-2 by a C. beijerinckii strain depended on the concentration of galactose. Galactose at 15 g/L provided the highest rate and yield of H-2 production, 117.45 mL of H-2/L.h and 2.02 mol of H-2/mole of galactose, respectively; the main metabolites from galactose fermentation were butyric acid (61%) and acetic acid (24%). Therefore, C. beijerinckii Br21 is a promising candidate for fermentative production of H-2 from galactose and algae biomass. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries
Grantee:María Eugenia Guazzaroni
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/06074-1 - Third-generation biohydrogen production from algae biomass and its hydrolysis derivatives
Grantee:Valeria Reginatto Spiller
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants