| Full text | |
| Author(s): |
Bernardo, Cassia Rubia
;
Silveira Camargo, Ana Vitoria
;
Ronchi, Luis Sergio
;
de Oliveira, Amanda Priscila
;
de Campos Junior, Eumildo
;
Borim, Aldenis Albaneze
;
Brandao de Mattos, Cinara Cassia
;
Bestetti, Reinaldo Bulgarelli
;
de Mattos, Luiz Carlos
Total Authors: 9
|
| Document type: | Journal article |
| Source: | INFECTION GENETICS AND EVOLUTION; v. 45, p. 170-175, NOV 2016. |
| Web of Science Citations: | 2 |
| Abstract | |
Chagas disease, caused by Trypanosoma cruzi, can affect the heart, esophagus and colon. The reasons that some patients develop different clinical forms or remain asymptomatic are unclear. It is believed that tissue immunogenetic markers influence the tropism of T. cruzi for different organs. ABO, Secretor and Lewis histo-blood group systems express a variety of tissue carbohydrate antigens that influence the susceptibility or resistance to diseases. This study aimed to examine the association of ABO, secretor and Lewis histo-blood systems with the clinical forms of Chagas disease. We enrolled 339 consecutive adult patients with chronic Chagas disease regardless of gender (cardiomyopathy: n = 154; megaesophagus: n = 119; megacolon: n = 66). The control group was composed by 488 healthy blood donors. IgG anti-T. cruzi antibodies were detected by ELISA. ABO and Lewis phenotypes were defined by standard hemagglutination tests. Secretor (FUT2) and Lewis (FUT3) genotypes, determined by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), were used to infer the correct histo-blood group antigens expressed in the gastrointestinal tract. The proportions between groups were compared using the chi(2) test with Yates correction and Fisher's exact test and the Odds Ratio (OR) and 95% Confidence Interval (95% CI) were calculated. An alpha error of 5% was considered significant with p-values <0.05 being corrected for multiple comparisons (pc). No statistically significant differences were found for the ABO (X-2: 2.635; p-value = 0.451), Secretor (X-2: 0.056; p-value = 0.812) or Lewis (X-2: 2.092; p-value = 0.351) histo-blood group phenotypes between patients and controls. However, B plus AB Secretor phenotypes were prevalent in pooled data from megaesophagus and megacolon patients (OR: 5.381; 95% CI: 1.230-23.529; p-value= 0.011; pc= 0.022) in comparison to A plus O Secretor phenotypes. The tissue antigen variability resulting from the combined action of ABO and Secretor histo-blood systems is associated with the digestive forms of Chagas disease. (C) 2016 Elsevier B. V. All rights reserved. (AU) | |
| FAPESP's process: | 11/08075-4 - Immunogenetic markers related to Chagas disease |
| Grantee: | Luiz Carlos de Mattos |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/05580-2 - Immunogenetics markers in Chagas disease |
| Grantee: | Cássia Rubia Bernardo |
| Support Opportunities: | Scholarships in Brazil - Master |