Validation of a new technique to detect Crytosporidium spp. oocysts in bovine feces

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Inacio, Sandra Valeria ; Gomesb, Jancarlo Ferreira ; Miranda Oliveira, Bruno Cesar ; Falcao, Alexandre Xavier ; Nagase Suzuki, Celso Tetsuo ; dos Santos, Bianca Martins ; Costa de Aquino, Monally Conceicao ; de Paula Ribeiro, Rafaela Silva ; de Assuncao, Danilla Mendes ; Freire Casemiro, Pamella Almeida ; Meireles, Marcelo Vasconcelos ; Saraiva Bresciani, Katia Denise

• Document type: Journal article
• Source: PREVENTIVE VETERINARY MEDICINE; v. 134, p. 1-5, NOV 1 2016.
• Web of Science Citations: 2

Abstract

Due to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp. oocysts, called TF-Test Coccidia, using fecal samples from calves from the city of Aragatuba, Sao Paulo. To confirm the aforementioned parasite and prove the diagnostic efficiency of the new technique, we used two established methodologies in the scientific literature: parasite concentration by centrifugal sedimentation and negative staining with malachite green (CSN-Malachite) and Nested-PCR. We observed good effectiveness of the TF-Test Coccidia technique, being statistically equivalent to CSN-Malachite. Thus, we verified the effectiveness of the TF-Test Coccidia parasitological technique for the detection of Cryptosporidium spp. oocysts and observed good concentration and morphology of the parasite, with a low amount of debris in the fecal smear. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 99/06228-4 - Development of new kits destined for the diagnosis of intestinal parasites in feces samples
• Grantee: Sumie Hoshino Shimizu
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE