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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of NOD2 and RIP2 in host-microbe interactions with Gram-negative bacteria: insights from the periodontal disease model

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Souza, Joao A. C. ; Medeiros, Marcell C. ; Rocha, Fernanda R. G. ; de Aquino, Sabrina G. ; Avila-Campos, Mario J. ; Spolidorio, Luis C. ; Zamboni, Dario S. ; Graves, Dana T. ; Rossa Junior, Carlos
Total Authors: 9
Document type: Journal article
Source: Innate Immunity; v. 22, n. 8, p. 598-611, NOV 2016.
Web of Science Citations: 3

NOD2 is a member of the NLR family of proteins that participate in the activation of the innate immune response. RIP2 is a downstream kinase activated by both NOD1 and NOD2. There is scarcity of information regarding the relevance of NOD2 in periodontitis, a chronic inflammatory condition characterized by inflammatory bone resorption. We used NOD2-KO and RIP2-KO mice in a model of microbial-induced periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans was injected in the gingival tissues three times/wk for 4 wk. Bone resorption was assessed by CT analysis; osteoclasts were identified by immunohistochemical staining for TRAP and inflammation was assessed using a severity score system in H/E-stained sections. Invitro studies using primary macrophages assessed the response macrophages using qPCR-based array and multi-ligand ELISA. Bone resorption and osteoclastogenesis were significantly reduced in NOD2-KO mice. Severity of inflammation was not affected. qPCR-focused arrays and multi-ligand ELISA showed that expression of pro-inflammatory mediators was reduced in NOD2- and RIP2-deficient cells. RANKL-induced osteoclastogenesis was impaired in NOD2- and RIP2-deficient macrophages. We conclude that NOD2 is important for osteoclast differentiation and inflammatory bone resorption invivo and also for the macrophage response to Gram-negative bacteria. (AU)

FAPESP's process: 10/05632-7 - Role of Nod proteins in the modulation of the immune response in periodontal diseases
Grantee:João Antonio Chaves de Souza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/05783-5 - Role of Nod proteins in the modulation of the immune response in periodontal diseases
Grantee:Carlos Rossa Junior
Support type: Regular Research Grants