| Full text | |
| Author(s): |
Fernandes, Lais Silva
;
Emerick, Guilhemie Luz
;
Ferreira, Rafaela Scalco
;
dos Santos, Neife Aparecida G.
;
dos Santos, Antonio Cardozo
Total Authors: 5
|
| Document type: | Journal article |
| Source: | TOXICOLOGY IN VITRO; v. 39, p. 84-92, MAR 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
Some organophosphorus compounds (OPs) induce a neurodegenerative disorder known as organophosphate-induced delayed neuropathy (OPIDN), which is related to irreversible inhibition of neuropathy target esterase (NTE) and impairment of neurite outgrowth. The present study addresses the effects of trichlorfon, mipafox (neuropathic model) and paraoxon (non-neuropathic model) on neurite outgrowth and neuroplasticity-related proteins in retinoic-acid-stimulated SH-SY5Y cells, a cellular model widely used to study the neurotoxicity of OPs. Mipafox (20 mu M) decreased cellular differentiation and the expression of neurofilament 200 (NF-200), growth associated-(GAP-43) and synaptic proteins (synapsin I and synaptophysin); whereas paraoxon (300 gM) induced no effect on cellular differentiation, but significant decrease of NF-200, GAP-43, synapsin I and synaptophysin as compared to controls. However, the effects of paraoxon on these proteins were significantly lower than the effects of mipafox. In conclusion, axonal cytoskeletal proteins, as well as axonal plasticity-related proteins are more effectively affected by neuropathic (mipafox) than by non-neuropathic (paraoxon) OPs, suggesting that they might play a role in the mechanism of OPIDN. At high concentration (1 mM), trichlorfon induced effects similar to those of the neuropathic OP, mipafox (20 mu M), but also caused high inhibition of AChE. Therefore, these effects are unlikely to occur in humans at non-lethal doses of trichlorfon. (C) 2016 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 12/16319-3 - In vitro study of the mechanisms of neurotoxicity of the organophosphate trichlorfon: strategies for neuroprotection |
| Grantee: | Lais da Silva Fernandes |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/26906-6 - Organophosphorus-induced delayed neuropathy: in vitro study of the mechanisms of neurotoxicity of trichlorfon and strategies of neuroprotection |
| Grantee: | Antonio Cardozo dos Santos |
| Support Opportunities: | Regular Research Grants |