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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intra-Articular Blockade of P2X7 Receptor Reduces the Articular Hyperalgesia and Inflammation in the Knee Joint Synovitis Especially in Female Rats

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Author(s):
Teixeira, Juliana Maia ; Dias, Elayne Vieira ; Parada, Carlos Amilcar ; Tambeli, Claudia Herrera
Total Authors: 4
Document type: Journal article
Source: JOURNAL OF PAIN; v. 18, n. 2, p. 132-143, FEB 2017.
Web of Science Citations: 9
Abstract

Synovitis is a key factor in joint disease pathophysiology, which affects a greater proportion of women than men. P2X7 receptor activation contributes to arthritis, but whether it plays a role in articular inflammatory pain in a sex-dependent manner is unknown. We investigated whether the P2X7 receptor blockade in the knee joint of male and female rats reduces the articular hyperalgesia and inflammation induced by a carrageenan knee joint synovitis model. Articular hyperalgesia was quantified using the rat knee joint incapacitation test and the knee joint inflammation, characterized by the concentration of cytokines tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and cytokine-induced neutrophil chemoattractant-1, and by neutrophil migration, was quantified using enzyme linked immunosorbent assay and by myeloperoxidase enzyme activity measurement, respectively. P2X7 receptor blockade by the articular coadministration of selective P2X7 receptor antagonist A740003 with carrageenan significantly reduced articular hyperalgesia, pro-inflammatory cytokine concentrations, and myeloperoxidase activity induced by carrageenan injection into the knee joint of male and estrus female rats. However, a lower dose of P2X7 receptor antagonist was sufficient to significantly induce the antihyperalgesic and anti-inflammatory effects in estrus female but not in male rats. These results suggest that P2X7 receptor activation by endogenous adenosine 5'-triphos-phate is essential to articular hyperalgesia and inflammation development in the knee joint of male and female rats. However, female rats are more responsive than male rats to the antihyperalgesic and anti-inflammatory effects induced by P2X7 receptor blockade. Perspective: P2X7 receptors could be promising therapeutic targets in the treatment of knee joint disease symptoms, especially in women, who are more affected than men by these conditions. (C) 2016 by the American Pain Society (AU)

FAPESP's process: 10/05381-4 - Involvement of P2X3 and P2X7 purinergic receptors in carrageenan-induced arthritis in rat's knee joint
Grantee:Claudia Herrera Tambeli
Support Opportunities: Regular Research Grants
FAPESP's process: 09/16854-3 - Involvement of P2X3 and P2X7 purinergic receptors in carrageenan-induced arthritis in knee joint of rats
Grantee:Juliana Maia Teixeira
Support Opportunities: Scholarships in Brazil - Doctorate