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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis?

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Author(s):
G. Peron [1] ; F.F. Fernandes [2] ; T.N. Landgraf [3] ; R. Martinez [4] ; A. Panunto-Castelo [5]
Total Authors: 5
Affiliation:
[1] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Biologia Estrutural e Funcional - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia - Brasil
[4] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Clínica Médica - Brasil
[5] Universidade de São Paulo. Faculdade de Filosofia Ciências e Letras de Ribeirão Preto. Departamento de Biologia - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 50, n. 4 2017-04-03.
Abstract

Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were positive to rPbHsp60, with 90.9% positivity to recombinant gp43 (rgp43). More importantly, sera from healthy subjects had 27% positivity to rPbHsp60 and none to rgp43. When rPbHsp60 was used in ELISA, we did not observe significant differences between the reactions with sera from PCM patients and healthy subjects, while the difference was clearly evident when the antigen was rgp43. Furthermore, rPbHsp60 was recognized by sera from patients with histoplasmosis, aspergillosis, sporotrichosis or tuberculosis in an ELISA test. These results show that rPbHsp60 is not a good antigen for PCM diagnosis. (AU)

FAPESP's process: 12/07924-0 - Determination of antibody titers against the recombinant protein HSP60 Paracoccidioides brasiliensis in sera from patients with different clinical forms of paracoccidioidomycosis
Grantee:Gabriela Peron
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/08552-0 - Identification of antigens from Paracoccidioides brasiliensis with immunotherapeutic potential for the treatment of paracoccidioidomycosis.
Grantee:Taise Natali Landgraf
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/03235-3 - Characterization, cloning and expression of a Paracoccidioides brasiliensis protein with suppressive activity of the immune response.
Grantee:Fabrício Freitas Fernandes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/12278-3 - Screening of Paracoccidioides brasiliensis antigens with potential immunotherapeutic application in paracoccidioidomicose
Grantee:Ademilson Panunto-Castelo
Support Opportunities: Regular Research Grants