| Full text | |
| Author(s): |
Romano, Renata Marino
;
Gomes, Samantha Nascimento
;
Scandolara Cardoso, Nathalia Carolina
;
Schiessl, Larissa
;
Romano, Marco Aurelio
;
Oliveira, Claudio Alvarenga
Total Authors: 6
|
| Document type: | Journal article |
| Source: | ENDOCRINE; v. 55, n. 2, p. 607-617, FEB 2017. |
| Web of Science Citations: | 9 |
| Abstract | |
The impact of thyroid hormone (TH) disorders on male reproductive biology has been a controversial issue for many years. Recently, we reported that hypothyroid male rats have a disruption of the seminiferous epithelium, which may compromise spermatogenesis. To improve the understanding of the reproductive pathogenesis of hypothyroidism and hyperthyroidism, male Wistar rats that developed these dysfunctions in adulthood were used as an experimental model. We evaluated the sperm production, reserves, transit time, morphology, and functionality (acrosome integrity, plasma membrane integrity, and mitochondrial activity), and the testicular expression of the TH receptors (Thra1 and Thra2, Thrb1, and Thrb2), deiodinases (Dio2 and Dio3), and the Mct8 transporter (Slc16a2) were assessed by reverse transcription followed by real-time quantitative PCR (RT-qPCR). The results were evaluated statistically by ANOVA and Tukey HSD test (P < 0.05). Hypothyroidism decreased the total and daily sperm productions and increased the sperm transit time through the epididymis, while the sperm functionality was reduced in both thyroid dysfunctions. Regarding the modulation of gene expression in the testis, hypothyroidism increased the expression of Thra1 and decreased the expression of Dio3, and hyperthyroidism increased the expression of Slc16a2. The observed alterations in spermatic production and function and in the expression of the TH receptor, deiodinase, and the TH transporter are suggestive of TH participation in spermatogenesis in adulthood. (AU) | |
| FAPESP's process: | 14/10845-0 - Consequences of hypothyroidism and thyrotoxicosis triggered in adulthood on male gonadal function: gene expression of molecular markers and proteomic profile in tissues of the hypothalamic-pituitary-gonadal axis |
| Grantee: | Claudio Alvarenga de Oliveira |
| Support Opportunities: | Regular Research Grants |