Advanced search
Start date
Betweenand
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunogenic potential of Rhipicephalus (Boophilus) microplus aquaporin 1 against Rhipicephalus sanguineus in domestic dogs

Full text
Author(s):
Patricia Martinez Évora [1] ; Gustavo Seron Sanches [2] ; Felix David Guerrero [3] ; Adalberto Pérez de León [4] ; Gervásio Henrique Bechara
Total Authors: 5
Affiliation:
[1] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[2] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[3] United States Department of Agriculture. Agricultural Research Service - Estados Unidos
[4] United States Department of Agriculture. Agricultural Research Service - Estados Unidos
Total Affiliations: 5
Document type: Journal article
Source: Revista Brasileira de Parasitologia Veterinária; v. 26, n. 1, p. 60-66, 2017-02-16.
Abstract

Abstract This study evaluated a recombinant aquaporin 1 protein of Rhipicephalus (Boophilus) microplus (RmAQP1) as antigen in a vaccine against R. sanguineus. Five dogs were immunized with RmAQP1 (10 µg) + adjuvant (Montanide) (G1), and five were inoculated with adjuvant only (G2), three times. Twenty-one days after the last immunization, animals of both groups were challenged with R. sanguineus larvae, nymphs and adults, and their biotic potential was compared. Blood samples were collected before each immunization and every 28 days after the last immunization for 10 weeks. Serum antibody titers (IgG) were assessed by ELISA. We observed that: engorgement period of adult females from G1 was 12% shorter than G2; larvae from G1 had 8.7% longer engorgement period than G2 and weighed 7.2% less; nymphs from G1 had 4.5% shorter engorgement period than G2 and weighed 3.6% less; although the antibody titers increased following the second immunization, they rapidly decreased after the third immunization. Results indicated low immunoprotection of RmAQP1 against adult R. sanguineus ticks, and possible efficacy on larvae and nymphs fed on immunized dogs. Further studies should be performed for a full evaluation of the immunoprotection of RmAQP1 against R. sanguineus infestations in dogs. (AU)

FAPESP's process: 13/09792-7 - EVALUATION OF THE IMMUNOGENIC POTENTIAL OF RHIPICEPHALUS (BOOPHILUS) MICROPLUS AQUAPORIN AGAINST THE RHIPICEPHALUS SANGUINEUS TICK, LATREILLE, 1806 IN DOMESTIC DOGS
Grantee:Patricia Martinez Évora
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/10394-6 - Immunogenic Potential of the Ribosomal Protein P0 Against the Tick Rhipicephalus sanguineus (Acari:Ixodidae)in Dogs
Grantee:Gustavo Seron Sanches
Support Opportunities: Scholarships in Brazil - Post-Doctoral