Immunogenic Potential of the Ribosomal Protein P0 Against the Tick Rhipicephalus sanguineus (Acari:Ixodidae)in Dogs

• Grant number: 13/10394-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2013
• End date: September 30, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Rosangela Zacarias Machado
• Grantee: Gustavo Seron Sanches
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The tick Rhipicephalus sanguineus has great medical and veterinary importance, since it acts as a vector of pathogens (viruses, bacteria, protozoa and nematodes), causing diseases in animals and in humans. The control of this tick has been mainly based on the use of chemical acaricides, however, the indiscriminate use has contributed to the selection of resistant ticks, in addition to high costs and environmental contamination. Vaccines have been considered a promising alternative for the control of ticks, although, to date, no commercial anti-tick vaccine available for use in dogs. With the advancement of molecular biology techniques, new molecules have emerged as candidates for vaccines for cattle ticks and potentially for dogs. Among these molecules highlights the ribosomal protein P0 of R. sanguineus and R. (B.) microplus. Recent studies have also shown the possibility of vaccines developed for a species of tick confer cross-protection for species genetically close. Thus, this study aims, in collaboration with the Centro de Ingeniería Genética y Biotecnologia de Havana - CIGB, Cuba, to evaluate the efficacy of peptide vaccines P0 against R. sanguineus in dogs in a challenge infestation through determination of the ticks biotic potential, histopathology of ticks fed on vaccinated hosts including the tick ultrastructure, histopathology of the tick attachment site, antibody anti-P0, and immunohistochemistry of unfed ticks and fed ticks on vaccinated hosts.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PATRICIA MARTINEZ ÉVORA; GUSTAVO SERON SANCHES; FELIX DAVID GUERRERO; ADALBERTO PÉREZ DE LEÓN; GERVÁSIO HENRIQUE BECHARA. Potencial imunogênico da aquaporina 1 de Rhipicephalus (Boophilus) microplus contra o carrapato Rhipicephalus sanguineus em cães domésticos. Revista Brasileira de Parasitologia Veterinária, v. 26, n. 1, p. 60-66, 2017-02-16. (13/09792-7, 13/10394-6)

RODRIGUEZ MALLON, ALINA; JAVIER GONZALEZ, LUIS; ENCINOSA GUZMAN, PEDRO ENRIQUE; HENRIQUE BECHARA, GERVASIO; SERON SANCHES, GUSTAVO; POUSA, SATOMY; CABRERA, GLEYSIN; CABRALES, ANIA; GARAY, HILDA; MEJIAS, RAUL; et al. Functional and Mass Spectrometric Evaluation of an Anti-Tick Antigen Based on the P0 Peptide Conjugated to Bm86 Protein. PATHOGENS, v. 9, n. 6, JUN 2020. (13/10394-6)

SANCHES, GUSTAVO S.; ANDRE, MARCOS R.; DO PRADO, ANGELO P.; ALLEGRETTI, SILMARA M.; REMEDIO, RAFAEL N.; NUNES, PABLO H.; MACHADO, ROSANGELA Z.; BECHARA, GERVASIO H.; CAMARGO-MATHIAS, MARIA I.. Oocyte maturation in the sloth's giant tick Amblyomma varium (Acari: Ixodidae) in an ecological context. Experimental and Applied Acarology, v. 64, n. 4, p. 519-531, DEC 2014. (10/00415-8, 13/10394-6)

SANCHES, GUSTAVO S.; EVORA, PATRICIA M.; MANGOLD, ATILIO J.; JITTAPALAPONG, SATTAPORN; RODRIGUEZ-MALLON, ALINA; GUZMAN, PEDRO E. E.; BECHARA, GERVASIO H.; CAMARGO-MATHIAS, MARIA I.. Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae). Veterinary Parasitology, v. 215, p. 78-87, JAN 15 2016. (10/00415-8, 13/10394-6)

EVORA, PATRICIA MARTINEZ; SANCHES, GUSTAVO SERON; GUERRERO, FELIX DAVID; DE LEON, ADALBERTO PEREZ; BECHARA, GERVASIO HENRIQUE. Immunogenic potential of Rhipicephalus (Boophilus) microplus aquaporin 1 against Rhipicephalus sanguineus in domestic dogs. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 26, n. 1, p. 7-pg., 2017-01-01. (13/09792-7, 13/10394-6)