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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of Three Sugarcane Homo/Homeologous Regions Suggests Independent Polyploidization Events of Saccharum officinarum and Saccharum spontaneum

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Vilela, Mariane de Mendonca ; Del Bem, Luiz Eduardo ; Van Sluys, Marie-Anne ; de Setta, Nathalia ; Kitajima, Joao Paulo ; Queiroga Cruz, Guilherme Marcelo ; Sforca, Danilo Augusto ; de Souza, Anete Pereira ; Gomes Ferreira, Paulo Cavalcanti ; Grativol, Clicia ; Cardoso-Silva, Claudio Benicio ; Vicentini, Renato ; Vincentz, Michel
Total Authors: 13
Document type: Journal article
Source: GENOME BIOLOGY AND EVOLUTION; v. 9, n. 2, p. 266-278, FEB 2017.
Web of Science Citations: 7
Abstract

Whole genome duplication has played an important role in plant evolution and diversification. Sugarcane is an important crop with a complex hybrid polyploid genome, for which the process of adaptation to polyploidy is still poorly understood. In order to improve our knowledge about sugarcane genome evolution and the homo/homeologous gene expression balance, we sequenced and analyzed 27 BACs (Bacterial Artificial Chromosome) of sugarcane R570 cultivar, containing the putative single-copy genes LFY (seven haplotypes), PHYC (four haplotypes), and TOR (seven haplotypes). Comparative genomic approaches showed that these sugarcane loci presented a high degree of conservation of gene content and collinearity (synteny) with sorghum and rice orthologous regions, but were invaded by transposable elements (TE). All the homo/homeologous haplotypesof LFY, PHYC, and TOR are likely tobe functional, because they are all under purifying selection (dN/dS << 1). However, they were found to participate in a nonequivalently manner to the overall expression of the corresponding gene. SNPs, indels, and amino acid substitutions allowed inferring the S. officinarum or S. spontaneum origin of the TOR haplotypes, which further led to the estimation that these two sugarcane ancestral species diverged between 2.5 and 3.5 Ma. In addition, analysis of shared TE insertions in TOR haplotypes suggested that two autopolyploidization may have occurred in the lineage that gave rise to S. officinarum, after its divergence from S. spontaneum. (AU)

FAPESP's process: 10/02610-2 - Allelic variation in sugarcane: quantification of sequence polymorphism
Grantee:Mariane de Mendonça Vilela
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)