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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments

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Author(s):
Kohatsu, Andrea A. N. ; Silva, Flavia A. J. ; Francisco, Acacio I. ; Rimoldi, Aline ; Silva, Marco T. A. ; Vargas, Maria D. ; da Rosa, Joao A. ; Cicarelli, Regina M. B.
Total Authors: 8
Document type: Journal article
Source: Brazilian Journal of Infectious Diseases; v. 21, n. 2, p. 125-132, MAR-APR 2017.
Web of Science Citations: 2
Abstract

Abstract Resistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed with ferrocenyl diamine hydrochlorides in six different strains of T. cruzi epimastigote forms (Y, Bolivia, SI1, SI8, QMII, and SIGR3). The last four strains have been recently isolated from triatominae and mammalian host (domestic cat). The expression of mitochondrial tryparedoxin peroxidase was analyzed by the Western blotting technique using polyclonal antibody anti mitochondrial tryparedoxin peroxidase obtained from a rabbit immunized with the mitochondrial tryparedoxin peroxidase recombinant protein. All the tested ferrocenyl diamine hydrochlorides were more cytotoxic than benznidazole. The expression of the 25.5 kDa polypeptide of mitochondrial tryparedoxin peroxidase did not increase in strains that were more resistant to the ferrocenyl compounds (SI8 and SIGR3). In addition, a 58 kDa polypeptide was also recognized in all strains. Ferrocenyl diamine hydrochlorides showed trypanocidal activity and the expression of 25.5 kDa mitochondrial tryparedoxin peroxidase is not necessarily increased in some T. cruzi strains. Most likely, other mechanisms, in addition to the over expression of this antioxidative enzyme, should be involved in the escape of parasites from cytotoxic oxidant agents. (AU)

FAPESP's process: 11/06525-2 - Evaluation of oxidative metabolism in Trypanosoma cruzi strains treated with diamines of ferrocene
Grantee:Regina Maria Barretto Cicarelli
Support Opportunities: Regular Research Grants