| Full text | |
| Author(s): |
Pirich, Cleverton Luiz
;
de Freitas, Rilton Alves
;
Torresi, Roberto Manuel
;
Picheth, Guilherme Fadel
;
Sierakowski, Maria Rita
Total Authors: 5
|
| Document type: | Journal article |
| Source: | BIOSENSORS & BIOELECTRONICS; v. 92, p. 47-53, JUN 15 2017. |
| Web of Science Citations: | 15 |
| Abstract | |
Low-cost piezoelectric devices, such as simple frequency monitoring quartz crystal microbalance (QCM) devices, have good clinical utility as fast diagnostic tools for the detection of several diseases. However, unspecific antigen recognition, poor molecular probe adsorption and the need for sample dilution are still common drawbacks that hinder their use in routine diagnosis. In this work, piezoelectric sensors were previously coated with thin films of bacterial cellulose nanocrystals (CN) to provide a more sensitive and adapted interface for the attachment of monoclonal immunoglobulin G (IgGNS1) and to favor specific detection of non-structural protein 1 (NS1) of dengue fever. The assembly of the immunochip surface was analyzed by atomic force microscopy (AFM) and the NS1 detection was followed by quartz crystal microbalance with (QCM-D) and without energy dissipation monitoring (QCM). The CN surface was able to immobilize 2.30 +/- 0.5 mg m(-2) of IgGNS1, as confirmed by AFM topography and phase images along with QCM-D. The system was able to detect the NS1 protein in serum with only 10-fold dilution in the range of 0.01-10 mu g mL(-1) by both QCM and QCM-D. The limits of detection of the two devices were 0.1 mu g for QCM-D and 0.32 mu g mL(-1) for QCM. As a result, QCM-D and QCM apparatuses can be used to follow NS1 recognition and have good potential for more sensitive, fast and/or less expensive diagnostic assays for dengue. (AU) | |
| FAPESP's process: | 09/53199-3 - Molecular recognition and energy storage: fundamental studies concerning geometry, size and synthesis effects on the optimization of the chemical properties of electroactive materials |
| Grantee: | Roberto Manuel Torresi |
| Support Opportunities: | Research Projects - Thematic Grants |