Hepatotoxicity and metabolic effects of cellular extract of cyanobacterium Radiocystis fernandoi containing microcystins RR and YR on neotropical fish (Hoplias malabaricus)

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Paulino, Marcelo Gustavo ; Rossi, Priscila Adriana ; Venturini, Francine Perri ; Tavares, Driele ; da Silva Souza, Naiara Elisabete ; Sakuragui, Marise Margareth ; Moraes, Gilberto ; Terezan, Ana Paula ; Fernandes, Joao Batista ; Giani, Alessandra ; Fernandes, Marisa Narciso

• Document type: Journal article
• Source: Chemosphere; v. 175, p. 431-439, MAY 2017.
• Web of Science Citations: 9

Abstract

The toxicological effect of cellular extract of cyanobacterium Radiocystis fernandoi strain R28 containing RR and YR microcystins was analyzed in the fish Hoplias malabaricus with emphasis on the liver structure and energetic metabolism, after short-term exposure. Fish were intraperitoneally (i.p.) injected with 100 mu g of equivalent MC-LR kg(-1) body mass containing in the cellular extract of R. fernandoi strain R28. Twelve and 96 h post-injection, the plasma, liver and white muscle were sampled for biochemical analyses and liver was also sampled for morphological analyses. After i.p. injection, the activity of acid phosphatase (ACP), alanine aminotransferase (ALT) and direct bilirubin increased in the plasma, while ALT and aspartate aminotransferase (AST) decreased in the liver. Glucose, lactate and pyruvate increased while protein decreased in the plasma; glycogen, pyruvate and lactate decreased in the liver; and glycogen and glucose increased in the muscle. Ammonia increased in the plasma, liver and muscle. The hepatocyte cell shape changed from polyhedral to round after cellular extract injection; there was loss of biliary canaliculus organization, but the biliary duct morphology was conserved in the liver parenchyma. In conclusion, microcystins present in the cellular extract of R. fernandoi strain R28 affect the liver structure of H. malabaricus, but the liver was able to continuously produce energy by adjusting its intermediate metabolism; glycogenolysis and gluconeogenesis maintained glucose homeostasis and energy supply. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/00728-1 - Effects of microcystins present in the crude extract of cyanobacterium, Microcystis aeruginosa, in the liver and muscle of traírão, Hoplias lacerdae.
• Grantee: Marcelo Gustavo Paulino
• Support Opportunities: Scholarships in Brazil - Doctorate