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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efferocytosis-induced prostaglandin E2 production impairs alveolar macrophage effector functions during Streptococcus pneumoniae infection

Full text
Author(s):
Salina, Ana C. G. ; Souza, Tais P. ; Serezani, Carlos H. ; Medeiros, Alexandra I.
Total Authors: 4
Document type: Journal article
Source: Innate Immunity; v. 23, n. 3, p. 219-227, APR 2017.
Web of Science Citations: 3
Abstract

Alveolar macrophages (AMs) are multitasking cells that maintain lung homeostasis by clearing apoptotic cells (efferocytosis) and performing antimicrobial effector functions. Different PRRs have been described to be involved in the binding and capture of non-opsonized Streptococcus pneumoniae, such as TLR-2, mannose receptor (MR) and scavenger receptors (SRs). However, the mechanism by which the ingestion of apoptotic cells negatively influences the clearance of non-opsonized S. pneumoniae remains to be determined. In this study, we evaluated whether the prostaglandin E2 (PGE(2)) produced during efferocytosis by AMs inhibits the ingestion and killing of non-opsonized S. pneumoniae. Resident AMs were pre-treated with an E prostanoid (EP) receptor antagonist, inhibitors of cyclooxygenase and protein kinase A (PKA), incubated with apoptotic Jurkat T cells, and then challenged with S. pneumoniae. Efferocytosis slightly decreased the phagocytosis of S. pneumoniae but greatly inhibited bacterial killing by AMs in a manner dependent on PGE(2) production, activation of the EP2-EP4/cAMP/PKA pathway and inhibition of H2O2 production. Our data suggest that the PGE(2) produced by AMs during efferocytosis inhibits H2O2 production and impairs the efficient clearance non-opsonized S. pneumoniae by EP2-EP4/cAMP/PKA pathway. (AU)

FAPESP's process: 11/23979-7 - Impact of eferocytosis in microbicidal activity of alveolar macrophages via receptors scavenger: role of prostaglandin E2
Grantee:Ana Carolina Guerta Salina
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/17611-7 - Effect of efferocytosis by dendritic cells on Th17 differentiation: role of Prostaglandin E2
Grantee:Alexandra Ivo de Medeiros
Support Opportunities: Research Grants - Young Investigators Grants