Nitric oxide coating polypropylene mesh increases angiogenesis and reduces inflammatory response and apoptosis

Author(s)

Prudente, Alessandro ; Favaro, Wagner Jose ; Reis, Leonardo Oliveira ; Zanettini Riccetto, Cassio Luis

• Document type: Journal article
• Source: INTERNATIONAL UROLOGY AND NEPHROLOGY; v. 49, n. 4, p. 597-605, APR 2017.
• Web of Science Citations: 3

Abstract

Objectives To evaluate the effect of implanted S-nitrosoglutathione (GSNO) coating polypropylene mesh in foreign-body response of rats. Methods Thirty female rats underwent to subcutaneous implant of five polypropylene (PP) fragments: uncoated PP (control); PP polyvinylalcohol (PVA) coated and PP PVA + GSNO (1, 10 and 70 mMol) coated. After euthanasia (4 and 30 days), eight slides were prepared from each animal: hematoxylin-eosin (inflammatory response); unstained (birefringence collagen evaluation); TUNEL technique (apoptosis); and five for immunohistochemical processing: CD-31 (angiogenesis), IL-1 and TNF-alpha (proinflammatory cytokynes), iNOS (NO synthesis) and MMP-2 (collagen metabolism). The inflammation area, birefringence index, apoptotic index, immunoreactivity and vessel density were objectively measured. Results Inflammatory reaction area at 4 days was 11.3, 15.2, 25.1, 17.1 and 19.3% of pure PP, PVA, GSNO 1, 10 and 70 mM, respectively, p = 0.0006 (PP x Others). At 30 days lower inflammatory area was observed in GSNO 10 and 70 mM compared to pure PP (5.3, 5.2 and 11.1%, respectively, p = 0.0001). Vessel density was higher for GSNO 1 mM (25.5%) compared to pure PP (19.6%) at 30 days only, p = 0.0081. Apoptotic index at 4 days was lower for GSNO 1 mM (49.3%) than pure PVA (60.6%), p = 0.0124. GSNO 10 and 70 mM reduced their apoptotic index at 30 days compared to 4 days (49.9 vs. 36.9 and 59.1 vs. 47.5%, respectively, p = 0.0397). Birefringence index, IL-1, TNF, MMP-2 and iNOS were not different. Conclusions Depending on concentrations, GSNO can increase angiogenesis, reduce inflammation and apoptosis compared to pure PP, without impact on cytokine, collagen organization/metabolism and endogenous NO synthesis. (AU)

• FAPESP's process: 11/11522-2 - Quantitative analysis and Immunohistochemistry evaluation of inflammatory reaction from monofilamentar polypropylene mesh coated with S-nitrosoglutathione-releasing poly(vinyl alcohol) film in grafted in subcutaneous rats
• Grantee: Cássio Luís Zanettini Riccetto
• Support Opportunities: Regular Research Grants