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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intrauterine Growth Restriction Programs the Hypothalamus of Adult Male Rats: Integrated Analysis of Proteomic and Metabolomic Data

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Pedroso, Amanda P. ; Souza, Adriana P. ; Dornellas, Ana P. S. ; Oyama, Lila M. ; Nascimento, Claudia M. O. ; Santos, Gianni M. S. ; Rosa, Jose C. ; Bertolla, Ricardo P. ; Kla-Witter, Jelena ; Christians, Uwe ; Tashima, Alexandre K. ; Ribeire, Eliane B.
Total Authors: 12
Document type: Journal article
Source: JOURNAL OF PROTEOME RESEARCH; v. 16, n. 4, p. 1515-1525, APR 2017.
Web of Science Citations: 9
Abstract

Programming of hypothalamic functions regulating energy homeostasis may play a role in intrauterine growth restriction (IUGR)-induced adulthood obesity. The present study investigated the effects of IUGR on the hypothalamus proteome and metabolome of adult rats submitted to 50% protein-energy restriction throughout pregnancy. Proteomic and metabolomic analyzes were performed by data independent acquisition mass spectrometry and multiple reaction monitoring, respectively. At age 4 months, the restricted rats showed elevated adiposity, increased leptin and signs of insulin resistance. 1356 proteins were identified and 348 quantified while 127 metabolites were quantified. The restricted hypothalamus showed down regulation of 36 proteins and 5 metabolites and up-regulation of 21 proteins and 9 metabolites: Integrated pathway analysis of the proteomics and metabolomics data indicated impairment of hypothalamic glucose metabolism, increased flux through the hexosamine pathway, deregulation of TCA cycle and the respiratory chain, and alterations in glutathione metabolism. The data suggest IUGR. modulation of energy metabolism and redox homeostasis in the hypothalamus of male adult rats: The present results indicated deleterious consequences of IUGR on hypothalamic pathways involved in pivotal physiological functions. These results provide guidance for future mechanistic studies assessing the role of intrauterine malnutrition in the development of metabolic diseases later in life. (AU)

FAPESP's process: 12/19321-9 - Proteomic and peptidomic characterization of Brazilian spider venoms by mass spectrometry
Grantee:Alexandre Keiji Tashima
Support Opportunities: Regular Research Grants
FAPESP's process: 10/20268-0 - Protein expression in the hypothalamus and adipose tissue of male and female rats exposed to intrauterine malnutrition
Grantee:Eliane Beraldi Ribeiro
Support Opportunities: Regular Research Grants