Metagenomic analysis of the microbiome in three different bioreactor configurations applied to commercial laundry wastewater treatment

Author(s)

Delforno, T. P. ; Lacerda, Jr., G. V. ; Sierra-Garcia, I. N. ; Okada, D. Y. ; Macedo, T. Z. ; Varesche, M. B. A. ; Oliveira, V. M.

• Document type: Journal article
• Source: Science of The Total Environment; v. 587, p. 389-398, JUN 1 2017.
• Web of Science Citations: 10

Abstract

The taxonomic and functional diversity of three different biological reactors (fluidized bed reactor, FBR; up-flow anaerobic sludge blanket reactor, UASB; and expanded granular sludge bed reactor, EGSB) used for commercial laundry wastewater treatment was investigated using metagenome shotgun sequencing. Metagenomes were sequenced on the Illumina Hiseq platform and were analyzed using MG-RAST, STAMP and PAST software. The EGSB and UASB reactors were more closely related based on taxonomic and functional profiles, likely due to similar granular sludge and procedures adopted to ensure anaerobic conditions. The EGSB and UASB reactors showed a predominance of methanogens and genes related to methanogenesis, with a prevalence of the acetoclastic pathway, in addition to the peripheral and central O-2-independent pathways for aromatic compound degradation. By contrast, FBR showed a dominance of aerobic microbiota and pathways for O-2-dependent aromatic compound degradation. Therefore, although the reactors showed similar surfactant removal levels, the microbial composition, functional diversity and aromatic compound degradation pathways were significantly distinct. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/16426-0 - Metagenomics of microbiota of anaerobic reactors applied in anionic surfactant degradation in commercial laundry wastewater
• Grantee: Tiago Palladino Delforno
• Support type: Scholarships in Brazil - Post-Doctorate