Electrophysiological properties and augmented catecholamine release from chromaffin cells of WKY and SHR rats contributing to the hypertension development elicited by chronic EtOH consumption

Author(s)

Souza Bomfim, Guilherme Henrique ; Mendez-Lopez, Iago ; Carlos Fernandez-Morales, Jose ; Fernando Padin, Juan ; Jurkiewicz, Aron ; Jurkiewicz, Neide Hyppolito ; Garcia Garcia, Antonio

• Document type: Journal article
• Source: European Journal of Pharmacology; v. 803, p. 65-77, MAY 15 2017.
• Web of Science Citations: 2

Abstract

It is known that chronic ethanol (EtOH) consumption leads to hypertension development and has been associated with deleterious effects on the cardiovascular system. Whether this condition alters calcium (Ca2+) signaling and exocytosis in adrenal chromaffin cells (CCs) as the case is for genetic hypertension, is unknown. We explored this question in four randomized experimental groups, male Wistar Kyoto (WKY/EtOH) and Spontaneously Hypertensive (SHR/EtOH) rats were subjected to the intake of increasing EtOH concentrations (5-20%, for 30 days) and their respective controls (WKY/Control and SHR/Control) received water. WKY/ EtOH developed hypertension and cardiac hypertrophy; blood aldehyde dehydrogenase (ALDH) and H(2)O(2)were also augmented. In comparison with WKY/Control, CCs from WKY/EtOH had the following features: (i) depolarization and higher frequency of spontaneous action potentials; (ii) decreased Ca2+ currents with slower inactivation; (iii) decreased K+ currents; (iv) augmented K+-elicited cytosolic Ca2+ transients ({[}Ca2+](c)); (v) enhanced K+-elicited catecholamine release. These cardiovascular, blood and CCs changes were qualitatively similar to those undergone by SHR/Control and SHR/EtOH. The results suggest that the hypertension elicited by chronic EtOH has pathogenic features common to genetic hypertension namely, augmented {[}Ca2+](c) transients and catecholamine release from their CCs. (AU)

• FAPESP's process: 14/01569-0 - Interference of drugs producing disturbances in the drug-receptor interactions in hypertension, hyperglycemia and neurotransmission emphasizing the modulator role of histamine
• Grantee: Neide Hyppolito Jurkiewicz
• Support Opportunities: Regular Research Grants