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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Positively Selected Sites at HCMV gB Furin Processing Region and Their Effects in Cleavage Efficiency

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Author(s):
Stangherlin, Lucas M. ; de Paula, Felipe N. ; Icimoto, Marcelo Y. ; Ruiz, Leonardo G. P. ; Nogueira, Mauricio L. ; Braz, Antonio S. K. ; Juliano, Luiz ; da Silva, Maria C. C.
Total Authors: 8
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 8, MAY 23 2017.
Web of Science Citations: 2
Abstract

Human cytomegalovirus is a ubiquitous infectious agent that affects mainly immunosuppressed, fetuses, and newborns. The virus has several polymorphic regions, in particular in the envelope glycoproteins. The UL55 gene encodes the glycoprotein B that has a variable region, containing a furin cleavage site and according to the variability different genotypes are characterized. Here we investigated variability and existence of selective pressure on the UL55 variable region containing the furin cleavage site in 213 clinical sequences from patients worldwide. We showed the occurrence of positive selective pressure on gB codons 461 and 462, near the furin cleavage site. Cleavage analysis of synthesized peptides demonstrated that most mutations confer better cleavage by furin, suggesting that evolution is acting in order to increase the efficiency cleavage and supporting the hypothesis that gB processing is important in the host. We also demonstrated that peptides containing sequences, that characterize genotypes gB2 and 3, are differentially cleaved by furin. Our data demonstrate for the first time that variability in the cleavage site is related to degree of gB processing by furin. (AU)

FAPESP's process: 13/14215-9 - Investigation of the role of human Cytomegalovirus in the resistance of glioblastoma cells to chemotherapy in vitro
Grantee:Maria Cristina Carlan da Silva
Support Opportunities: Regular Research Grants