Beta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic forms

Author(s)

Macedo, Silene ; Ferreira Rodrigues, Joao Paulo ; Schenkman, Sergio ; Yoshida, Nobuko

• Document type: Journal article
• Source: Microbes and Infection; v. 19, n. 4-5, p. 295-301, APR-MAY 2017.
• Web of Science Citations: 2

Abstract

The involvement of beta-adrenergic receptor (beta-AR) in host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is not known. We examined whether isoproterenol, an agonist of beta-AR, or nonselective beta-blocker propranolol affected MT internalization mediated the stage-specific surface molecule gp82. Treatment of HeLa cells with propranolol significantly inhibited MT invasion whereas isoproterenol had no effect. Propranolol, but not isoproterenol, also inhibited the lysosome spreading required for gp82-dependent MT invasion. The effect of propranolol in inhibiting MT internalization was not due to the prevention of gp82 interaction with beta-AR. It was mainly associated with its ability to impair lysosome spreading. (C) 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

• FAPESP's process: 11/51475-3 - Molecular and cellular biology of the parasitism by Trypanosoma cruzi
• Grantee: José Franco da Silveira Filho
• Support Opportunities: Research Projects - Thematic Grants